芥菜子对DMH诱导大鼠大肠癌预防作用的体内研究

Prevention effect of mustard seed on colorectal cancer in rats induced by DMH in vivo

目的:探讨芥菜籽(MS)对1,2-二甲基肼(DMH)诱导的大鼠大肠癌的预防作用。方法:用DMH腹腔注射诱导大鼠发生大肠癌,观察MS对诱癌率及胸腺指数和脾指数等的影响。结果:用DMH成功诱导大鼠大肠癌模型,正常对照组未见肿瘤发生,DMH组腺癌发生率为83.33%(10/12),腺瘤发生率为16.67%(2/12),总肿瘤发生率为100%(12/12),肝及淋巴结转移率为58.33%(7/12)。5%MS和7.5%MS组腺癌发生率比DMH组分别降低了58.33%和75.00%(χ2=15.662,P=0.000),肝及淋巴结转移率分别降低了41.66%和50.00%(χ2=8.585,P=0.014),总肿瘤发生率〔66.67%(8/12),41.70%(5/12)〕亦显著低于DMH组(χ2=9.687,P=0.008),而腺瘤发生率〔41.67%(5/12),33.33%(4/12)〕则高于DMH组。7.5%MS组大鼠胸腺指数和脾指数均明显大于DMH组,P值分别为0.013和0.037;同时5%MS组胸腺指数也显著大于DMH组,P=0.039。结论:MS对DMH诱导的大肠癌有预防发生及减少转移的作用,并能改善机体免疫功能。

OBJECTIVE： To observe the effect of mustard seed （MS） against colorectal cancer in rats induced by 1, 2-dimethylhydrazine （DMH） in vivo. METHODS： The eoloreetal cancer model induced by DMH intraperitoneal injection. The effect of mustard seed on the incidence of eolorectal cancer, spleen index and thymus index were observed. RESULTS： The eolorectal cancer model was successfully established in this experiment. The incidence of colorectal adenocarcinoma in DMH group was 83. 33% （10/12）, the adenoma incidence was 16. 67% （2/12）, the total tumor incidence was 100% （12/12）, and the liver and mesenterie lymph node metastasis rate was 58.33 % （7/12）. The incidence of adenoeareinoma in 5% MS group and 7.5%MS group was 58.33% and 75.00% respectively lower than that in DMH group （？（2 =15. 662, P=0. 000）. The liver and mesenteric lymph node metastasis rate of colorectal cancer in 5% MS group and 7. 5%MS group was 41.66% and 50.00% respectively lower than that in DMH group （ 2 =8. 585,P 0. 014）. And the incidence of total colorectal tumor in 5% MS group and 7.5%MS group was 66.67%（8/12） and 41.70%（5/12） respectively lower than that in DMH group （X2）C =9. 687,P=0. 008）. While the incidence of colorectal adenoma in 5% MS group （41.67%, 5/12） and 7. 5 % MS group （33.33%,4/12） was slightly higher than that in DMH group （16. 67%,2/12）. The thymus and spleen index of rats in 7.5%MS group were markedly higher than that in DMH group （P=0. 013; P=0. 037）. And the thymus index of rats in 5%MS group was also markedly higher than that of DMH group （P=0. 039）. CONCLUSION： MS have the effects of preventing colorectal cancer induced by DMH, and it can improve organisn＇/s immunefunetion.