兔梨状隐窝VX2瘤模型的建立及解剖与影像学观察

Establishment of rabbit model with VX2 cell pyriformsinus grafting tumor

目的建立兔梨状隐窝VX2移植瘤模型。方法先以VX2细胞在新西兰大白兔制备移植瘤并传代,再将兔移植瘤制备组织悬液(组织块约1mm^3大小,约含1～2×10~6/m1个细胞),直达喉镜下接种于17只兔梨状隐窝,然后行影像学观察及瘤体组织学鉴定,分析成瘤率及生长特性。结果移植瘤组织悬液2～4周后,影像检查及瘤体解剖证实,16只兔梨状隐窝内形成了移植瘤,成瘤率为94.1%,但90%荷瘤兔在4周内死亡。结论 VX2细胞移植瘤组织制备瘤组织悬液、直达喉镜下接种于梨状隐窝的方法 ,能够成功制备兔喉咽癌移植瘤模型。

Objeetive To establish a rabbit model with pyriformsinus carcinoma （PSC） grown from grafted VX2 tumor cells to serve as an animal research tool system for experimental study of hypolaryngeal carcinoma. Methods Grafting tumor was prepared with VX2 tumour cells among New Zealand rabbits for several generations at first, and then, grafting tumor was taken made into a suspension with the tissue pieces at a size of 1 mm3 or so （in the cell density about 1-2× 10^6/ml cells）. After that, the tissue suspension was injected into pyriformsinus mucosa of 17 rabbits under direct laryngoscope to grow PSC. Following tumor suspension injected, all these rabbits were given CT scanning regularly to detect the growing status, and tumor tissues were collected to do histological assays to identify if the grafting tumor model of VX2 cells established successhxlly based on the analysis of tulnor growing rate and their behaviour characteristics. Results Two to four weeks late following the tumor tissue suspension injected, Grafting tumors were successfully grown in piriform recess among 16 rabbits, as confirmed by CT images and histological evidences meaning that the model was prepared successfidly. The growing rate of grafting tumor was 94.1% （16/17）, while 90% of the animals with PSC died in 4 weeks following tumour cells grafting. Conclusions It is suggested that the preparing procedures for PSC model are practicable by using tissue suspension, made from the grafting tumors of VX2 cells prepared among Zealand rabbits at first, injecting into the mucosa of piriform recess of rabbits under direct laryngoscope. This kind of animal model can be utilized in the research field of hypolaryngeal carcinoma.