α干扰素诱导人鼻咽癌细胞凋亡及其机制探讨

Interferon Alpha Induced Apoptosis in Human Nasopharyngeal Carcinoma Cell Line CNE2 and Its Possible Mechanism

目的:观察α干扰素对鼻咽癌细胞株CNE2的体外生长抑制和诱导凋亡作用,为其在抗肿瘤治疗方面的应用提供理论依据。方法:α干扰素治疗组和未治疗组分别采用免疫印记检测磷酸化AKT和caspase-3蛋白水平、XTT检测癌细胞生长和流式细胞术分析癌细胞凋亡。结果:α干扰素与CNE2细胞株共同培养48h后,磷酸化AKT蛋白水平明显低于CNE2细胞组,而caspase-3蛋白水平明显高于CNE2组;XTT试验显示α干扰素明显抑制CNE2细胞系的的生长,这种抑制作用呈现时间和浓度依赖性;AnnexinV/PI双染流式细胞检测α干扰素与CNE2细胞系共育48h后细胞的早期凋亡率23.42%,而对照组的早期凋亡率仅为4.5%(P<0.05)。结论:α干扰素诱导鼻咽癌细胞凋亡,从而抑制其体外生长增殖,具有抗肿瘤作用。

Objective： To study the effects of interferon α（IFNα） on proliferation and apoptosis of human nasopharyngeal carcinoma cell line CNE2.Methods： Protein expression of caspase-3 and pAKT were analyzed by Western blots in two groups.The inhibition of proliferation of IFNα in CNE2 cells was estimated by XTT assay.Flow cytometry was used to examine the rate of apoptosis.Results： Protein levels for pAkt was significantly lower in CNE2 cell line after treatment with IFNα than in the control group.The increased expression of caspase-3 was observed in CNE2 cell line.The proliferation of CNE2 cells was significantly inhibited by IFNα in a dose-dependent and time-dependent manner.Flow cytometry（AnnexinV/PI） indicated that the rate of apoptiosis early were respectively 23.42% and 4.5% in IFNα group and control group（P0.05）.Conclusion： IFNα could inhibit the proliferation of the human nasopharyngeal carcinoma cell line CNE2 and induce apoptiosis,and has direct anti-tumor effects on human nasopharyngeal carcinoma cell.