NS复合乳酸菌制剂对断奶仔猪免疫水平的影响

Effects of NS-Lactobacillus Complex on Immunity Levels in Weaning Piglets

选择二元杂交(杜/长)20日龄断奶仔猪90头,平均断奶体重8.99kg±1.60kg,按体重和性别分成3个处理组,每处理3个重复。A组为对照组,饲喂玉米、豆粕基础日粮;B组为基础日粮添加NS复合乳酸菌组2g/kg;C组为基础日粮添加抗生素组(2g/kg金霉素、0.25g/kg阿莫西林和0.125g/kg的支原净)。试验共30d,研究微生态制剂NS复合乳酸菌对断奶仔猪体液免疫和细胞免疫水平的影响。结果表明,试验30d时,NS复合乳酸菌组与对照组相比,断奶仔猪血清中的IgM含量和干扰素INF-γ的含量分别高出19.7%和10.2%,猪瘟病毒抗体阳性率高出33.3%,差异显著(P<0.05);与NS复合乳酸菌组相比,抗生素组仔猪外周血T细胞CD 8+亚群的比例要高出79.2%,差异显著(P<0.05)。NS复合乳酸菌制剂对加强仔猪体液免疫,维持仔猪的猪瘟、伪狂犬病毒抗体水平和促进机体细胞免疫具有良好效果。

A total of 90 crossbred piglets （1/2 Large white × 1/2 Duroc, 20-day-old, 8.99 kg±1. 60 kg ） were divided into 3 groups by weight and gender, with 3 replicates in each group and 10 piglets in each replicate. The 3 groups were as follows, control group （fed corn-soybean basal diet）, NS-Lactobacillus group （added 2 g/kg NS-Lactobacillus complex in basal diet）, antibiotic group （added 2 g/kg aureomycin, 0.25 g/kg amoxicillin and 0. 125 g/kg tiamulin in basal diet）. The experiment time was 30 days. The humoral immunity, T lymphocyte subpopulations and the antibody levels of classical swine fever virus （CSFV） and pseudorabies virus （PRV） were assayed. The results showed as follows, the levels of IgM, IFN-y and the CSFV antibody positive rate in blood in NS-Lactobacillus group were increased by 19.7%, 10.2% and 33.3% compared with control group （P〈0.05）. T lymphocyte CD8＋ subpopulations in antibiotic group increased 79.2% compared with NS-Lactobacillus group （P〈0.05）. These suggested that NS-Lactobacillus complex could improve humoral immunity, maintain the antibody levels of CSFV and PRV and increase cellular immunity of piglets.