小鼠抗鹅细小病毒VP3蛋白单克隆抗体的制备

Preparation of Monoclonal Antibody Against VP3 Protein of Goose Parvovirus

采用杂交瘤技术,用纯化的鹅细小病毒(Goose parvovirus,GPV)VP3蛋白免疫Balb/c小鼠制备单克隆抗体(mAb),经间接ELISA方法筛选和有限稀释法进行3次亚克隆后获得一株抗鹅细小病毒VP3蛋白杂交瘤细胞株(4E5)。经鉴定,该mAb为IgG1亚型,轻链为κ链。Western blot试验结果表明,获得的4E5mAb可特异性地识别鹅细小病毒VP3蛋白。本试验成功制备了抗鹅细小病毒VP3蛋白单克隆抗体,为研究鹅细小病毒病诊断方法奠定了基础。

Balb/c mice were immunized with purified goose parvovirus（GPV） VP3 to prepare the monoclonal antibody. The anti-GPV hybridoma cells（4ES）were prepared with hybridoma technique, indirect ELISA and limiting dilution assay for 3 times were used to obtain the monoclonal antibody hybridoma cells. After identification, the MAb is IgG1 subtype and the light chain is k. The results of Western blot showed that GPV VP3 protein could be specially recognized by 4E5 MAb. The anti-GPV VP3 monoclonal antibody was prepared successfully,which lay a foundation for diagnosis and treatment of GPV.