PKA和PKC系统对小鼠精原细胞增殖的影响

Effects of PKA and PKC on proliferation of mouse spermatogonial cells in vitro

利用精原细胞-体细胞体外无血清共培养模型研究了蛋白激酶A(PKA)和蛋白激酶C(PKC)系统对小鼠A型精原细胞增殖的影响。结果表明,PKA的激活剂forskolin(FSK)可显著刺激小鼠A型精原细胞增殖,这一作用可被PKA抑制剂H89所抑制;PKC的激活剂phorbol-12-myristate-13-acetate(PMA)也具有促进A型精原细胞增殖的作用,这一作用可被PKC的抑制剂H7所抑制。说明PKA和PKC信号通路在小鼠A型精原细胞的发育过程中发挥着重要的调控作用,激活PKA和PKC信号转导途径能够促进A型精原细胞的增殖。

The serum-free spermatogonium-somatic cell coculture model was chosen to evaluate the effects of activation of protein kinases A（PKA） and protein kinases C（PKC） on proliferation of mouse type A spermatogonial cells.It was found that forskolin（FSK）,an activator of PKA,exerted proliferating action on type A spermatogonia at the concentration of 10-6-10-5 mol·L-1.Meanwhile,PKA inhibitor H89 inhibited this effect.Likewise,Activation of PKC by Phorbol 12-myristate 13-acetate（PMA） elicited proliferation on spermatogonia at 10-8 to 10-7 mol·L-1 and PKC inhibitor H7 inhibited the effect.These suggests the activation of PKC and PKA on type A spermatogonial proliferation.