模拟飞行对内皮细胞损伤机制研究

Mechanism of simulated flight underlying injury of endothelial cells

目的探讨模拟飞行对主动脉内皮细胞损伤的影响机制。方法将16只雄性新西兰白兔随机分为对照组和模型组。模拟飞行内皮细胞模型由离心机训练建立。ELISA法检测细胞悬液血红素氧化酶-1(HO-1)及内皮素-1(ET-1)含量;测定细胞悬液丙二醛(MDA)、超氧化物歧化酶(SOD)。结果与空白对照组相比,模拟飞行组的主动脉内皮细胞SOD活性显著降低(P<0.05);MDA含量显著增加(P<0.05)。与空白对照组相比,模拟飞行组的主动脉内皮细胞HO-1含量增高(P<0.05);ET含量显著增加(P<0.05)。结论模拟飞行可能通过氧化应激及释放血管活性物质ET-1,诱导内皮细胞损伤;而HO-1可能起到自我保护作用。

Objective To study the mechanism of simulated flight underlying injury of aortic endothelial cells. Methods Sixteen male New Zealand rabbits were randomly divided into control group and model group, 8 in each group. A simulated flight model of endothelial cells was established by centrifuge training. Levels of heme oxygenase-l（HO-1）, endothelin-l（ET-1）, molonyldialdehyde（MDA） and superoxide dismutase（SOD） in cell suspension were measured by ELISA. Results The activity of SOD in aortic endothelial cells was significantly lower, while the levels of MDA, HO-1 and ET-1 were significantly higher in model group than in control group（P〈0.05）. Conclusion Simulated flight can induce injury of endothelial cells by increasing oxidative stress and releasing ET-1, while HO-1 may have a self protective effect.