帕金森病患者脑脊液蛋白质组学的研究

Cerebrospinal fluid in patients with Parkinson's disease:a proteomic research

目的:通过脑脊液蛋白质组学实验技术体系,筛选并鉴定帕金森病患者脑脊液特异蛋白,初步探讨帕金森病的发病机制。方法:选自广州医学院第一附属医院神经内科与脑深部电刺激术(deep brainstimulation,DBS)治疗中心帕金森病患者和对照组脑脊液为研究对象,进行双向凝胶电泳,经银染、图像分析寻找差异蛋白质,并进行质谱分析鉴定。结果:鉴定得到4种差异蛋白质,分别是CENP-E、Tetranectin非钙离子C型凝集素样结构域、假想蛋白FLJ38159和KIAA1640。帕金森病组中KIAA1640蛋白含量占总蛋白含量的比例(0.09±0.05)%高于对照组(0.03±0.00)%,CENP-E(0.04±0.02)%、Tetranectin非钙离子C型凝集素样结构域(0.04±0.02)%、假想蛋白FLJ38159(0.11±0.06)%蛋白含量均低于对照组[(0.08±0.01)%,(0.07±0.02)%,(0.17±0.04)%],P<0.05。结论:本研究鉴定出4种差异蛋白,这些蛋白可能参与帕金森病的发病,但具体机制及其在帕金森病发病中的作用有待进一步研究。

Objective：Proteomics technology were applied to screen and identify disease-specific protein in eerebrospinal fluid （CSF） of patients with Parkinson＇s disease （PD）, as an attempt to explore the pathogenesis of PD. Methods： The cerebrospinal fluid of PD patients and control CSF were collected at the Department of Neurology and Deep Brain Stimulation（DBS） Center of First Affiliated Hospital of Guangzhou Medical University. Two-dimensional gel electrophoresis with silver staining and subsequent image analysis was performed to screen for differential proteins which were then identify with mass spectrometry. Results：Four differential proteins were identified by mass spectrometry ： CENP-E, calcium-free form of the C-type lectin-like domain of tetranectin, and two hypothetical proteins （FLJ38159 and KIAA1640）. The PD group showed higher content of KIAA1640 protein [ （0.09 ±0.05 ） % vs （ 0. 03 ±0. 00） % ] as calculated by percentage of total proteins, lower contents of C ENP-E protein[ （0.04 ± 0.02）% vs （0.08 ±0.01 ）% ], calcium-free form of the C-type lectin-like domain of tetranectin [ （0. 04 ±0.02）% vs （0. 07 ±0. 02 ）% ] and hypothetical protein FLJ38159 [ （0.11 ±0. 06 ）% vs （0.17 ±0.04） % ] compared with the control group （ all P 〈 0.05 ）. Conclusion ： The four types of differential proteins identified in this study may be involved in the pathogenesis of Parkinson＇s disease, although their specific mechanisms and roles remain to be further studied.