影响木薯再生系统的几个因素的研究

Studies on Influenc ing Factors of Cassava Regeneration System

以木薯7个优良品种SC6,SC7,SC8,SC124,NZ188,C3以及C4为实验材料,对木薯再生体系中的几个重要影响因素进行了研究。结果表明:6-BA对抑制木薯的顶端优势有明显的效果,浓度越高抑制作用越强;2,4-D和picloram能促进木薯初级体胚的形成,两者效果相当,在初级体胚诱导过程中,所有品种的产胚率都能达到100%;在初级体胚继代过程中,SC7产胚率很低,其他品种初级体胚继代产胚率都达到90%以上;体胚成熟时间对木薯子叶器官发生影响很大,成熟2周左右的子叶器官发生率较高,诱导频率达到70%以上;基因型不同的木薯的体细胞胚发生频率和成熟子叶胚器官发生频率差异较大,其中SC8最高,NZ188次之,SC7最低;在培养基中,加入5mg·L-1的AgNO3能提高成熟子叶胚的器官发生能力。

In this report, Varieties of SC6, SC7, SC8, SC124, NZ188, C3 and C4 were used as experimental materials, several significant factors which influence the regeneration system of cassava were studied. The results indicated that 6 - BA had a significant inhibitory effect on apical dominance of cassava, the higher the concen- tration was, the stronger the inhibition effects was ; 2,4 - D and picloram had almost equal effects for promoting somatic embryogenesis, in the process of induction of primary somatic embryos, embryogenesis rate of all breeds reached 100%, except SC7 remained a low embryogenesis rate of secondary somatic embryos, embryogenesis rate of secondary somatic embryos of other varieties went up to 90% ; the ripening time of somatic embryos had a great influence on the organogenesis of cotyledon, frequency of cotyledon organogenesis was higher around 2 weeks after embryo maturation and the induction frequency could be more than 70% ; the somatic embryogenesis ratio and cotyledon var had the highest addition of AgNO3 （ ledonarv. organogenesis frequencies were very different among distinct genotypes,SC8 and NZ188 cuhi- and second- 5 mg · L^-1） highest frequency respectively, SC7 cultivar displayed the lowest frequency; The into subculture could help improving the organogenesis capacity of mature cotyledonary.