转基因玉米MON810基体标准物质Q—PCR量值协同实验研究

Cooperative Verification of the Value of GM Maize MON810 Matrix Reference Materials with Q-PCR Method

针对3种不同转基因含量水平的转基因玉米MON810基体标准物质，组织7家实验室对其量值采用实时荧光定量PCR（Q-PCR）方法进行了协同实验研究，并对Q-PCR得到的参考值进行了不确定度分析。经过加权平均，转基因玉米MONS10A、B、C3个水平的基体标准物质转基因含量的Q—PCR方法参考值分别为0．51％±0．10％、1．1％±0．2％、4．7％±0．7％（k=2）。

For 3 kinds of maize matrix reference materials with different genetically modified MON810 maize contents, a multi-laboratory verification for their Q-PCR values is organized. The uncertainties of the multi-laboratory values are evaluated. By weighted average, the Q-PCR values and uncertainties of A, B and C levels of the genetically modified MON810 maize matrix reference materials are 0. 51% ±0. 10% , 1. 1%±0. 2% , 4. 7%±0.7% （k =2）, respectively.