摘要
对转基因油菜T45荧光定量PCR方法的关键参数进行了方法验证，结果表明该方法适合于标准物质协同实验。选择6家实验室采用经过验证的方法对转基因油菜T45质粒分子标准物质进行了协同实验研究。通过比较分析转基因油菜T45基因DNA与质粒分子DNA两者产生的标准曲线的斜率（扩增效率）、线性拟合度和截距，发现转基因油菜T45基因组DNA与质粒分子DNA具有相似的扩增效率和线性相关系数。经统计学分析各参与实验室的数据等精度，转基因油菜T45质粒分子标准物质的标准值和扩展不确定度为0．98±0．02（％=2）。
The key parameters of the real time PCR method for genetically modified canola T45 were validated , the results show that the real time PCR method can be used to co-validate the reference value for GM canola T45 reference material. Six laboratories are chosen to characterize the property values of GM T45 plasmid reference materials with the same method. The slop （PCR amplification efficiency）, intercept and linearity coefficient of GM 3＂45 genomic DNA and plasmid DNA are analyzed. The results show that the PCR amplification efficiency and linearity coefficient between genomic DNA and plasmid DNA are not significantly different. The statistic test shows the data from different labs are equal. The property value and expanded uncertainties for GM canola T45 plasmid reference material is 0. 98 ±0. 02 （k = 2）.
出处
《计量学报》
CSCD
北大核心
2011年第B12期119-123,共5页
Acta Metrologica Sinica
基金
国家转基因生物新品种培育科技重大专项（2008ZX（18012-003）
科技支撑项目（2008BAK41801）
