猪肺炎支原体的分离方法

Ways to isolate Mycoplasma hyopneumoniae

为了寻求分离猪肺炎支原体更好的方法,本试验采用剪碎法、研磨法、匀浆法和灌洗法对猪肺组织进行处理,并研究了不同肺脏组织处理方法对猪肺炎支原体活力的影响。结果显示,剪碎法与灌洗法获得的处理液对猪肺炎支原体的影响小于研磨法与匀浆法。采用剪碎法、灌洗法和研磨法对100份猪肺组织进行猪肺炎支原体野毒株分离的结果显示,经PCR鉴定,剪碎法分离到1株猪肺炎支原体,分离率为1%;研磨法分离到7株猪肺炎支原体,分离率为7%;灌洗法分离到2株猪肺炎支原体,分离率为2%。以上结果表明,研磨法获得的组织处理液对支原体活力有一定的影响,但其分离率好于剪碎法和灌洗法。因此,研磨法是一种较好的猪肺炎支原体分离方法。

Four methods（shearing directly by scissors,grinding by mortar,stirring by homogenate machine,and lavaging from bronchus）were designed to deal with swine lung tissue to study the effects of treating methods on the isolation of Mycoplasma hyopneumoniae.The results showed that the methods of shearing and lavaging had less influence on the activity of Mycoplasma hyopneumoniae than the methods of grinding and stiring.Three methods of shearing,lavaging and grinding were used for the isolation experiment of 100 lung tissues samples.One virulent strain was obtained by the method of shearing,with the isolation rate of 1%.Seven strains were obtained by the method of grinding,with the isolation rate of 7%.Two strains was obtained by the method of lavaging,with the isolation rate of 2%.The results indicated that the method of grinding for isolation and culture of Mycoplasma hyopneumoniae was better than the methods of lavaging and shearing.The method of grinding offered a fairly good reference.