白细胞介素10和白细胞介素6在淋巴瘤中的表达及其意义

Significance of IL-IO and IL-6 expression in lymphoma

目的探讨白细胞介素（IL）-10和IL-6在不同类型淋巴瘤组织与血浆中的表达及其意义。方法收集97例淋巴瘤患者石蜡组织和血浆标本，应用免疫组织化学和酶联免疫吸附试验（ELISA）的方法观察组织与血浆中IL-10和IL-6表达情况。结果IL-10和IL-6在不同类型淋巴瘤组织中皆呈阳性表达，且各亚型之间差异无统计学意义（x2=0．815，x2=0．542，均P〉0．05）；IL-10和IL-6分别在肿瘤中的巨噬细胞和血管内皮细胞也有不同程度的阳性表达。淋巴瘤患者血浆IL-10和IL-6水平[（232．57±191．59）pg／ml、（80．70±89．68）Pg／ml]高于健康对照组[（59．12±68．35）Pg／ml、（45．68±33．66）pg／ml]，差异有统计学意义（t=6．968，t=2．896，P〈0．05）；血浆IL-10和IL-6水平随肿瘤细胞表达强度增强而增强（x2=0．815，x2=0．542，P〈0．05）；IL-10和IL-6在淋巴瘤细胞表达强度两者呈正相关（L=0．394，P〈0．05）。结论IL-10和IL-6在淋巴瘤患者淋巴瘤组织和血浆中都有表达，淋巴瘤患者血浆IL—10和IL-6水平高于健康对照组，二者在淋巴瘤的发生、发展中起着一定的作用，可能作为淋巴瘤诊断的辅助指标。

Objective To investigate the expression of IL-10 and IL-6 in tissue and plasma of subtypes lymphoma and its significance. Methods Immunohistochemical technique and enzyme-linked immunosorbent assay （ELISA） were used to assess the expression of IL-10 and IL-6 in 97 cases paraffin tissue and plasma samples from different subtypes of lymphoma. Results Expression of IL-10 and IL-6 was positive in all lymphoma tissue samples, but there is no significant difference among different subtypes lymphoma （x2 = 0.815, X2 = 0.542, P 〉 0.05）. Tumor samples were found positive IL-10 and IL-6 expression in macrophages and vascular endothelial cells by immunohistochemistry respectively. Expression of IL-10 and IL-6 in plasma of lymphoma were higher than that in control respectively [（232.57±191.59） pg/ml vs （59.12± 68.35） pg/ml, （80.70±89.68） pg/ml vs （45.68±33.66） pg/ml], with significant difference （t = 6.968, t = 2.896, P 〈0.05）. IL-10 and IL-6 plasma levels increased with enhanced expression in lymphoma （X2 = 0.815, X2 = 0.542, P 〈 0.05）. Which was found to be correlated with each other （r, = 0.394, P 〈 0.05）. Conclusion The expression of IL-10 and IL-6 had been found at various degrees in tissues and plasma of lymphoma. IL-10 and IL-6 in plasma of lymphoma were higher than that in controls. These results showed that IL-10 and IL-6 may cooperate in vivo with lymphoma cell proliferation and infiltrate. IL-6 and IL-10 maybe served as useful indicators for diagnosis of lymphoma.