S-100B蛋白及血管内皮生长因子测定在病毒性脑炎诊断中的意义

Significance of S-IOOB protein and vascular endothelial growth factor in the diagnosis of viral encephalitis

目的通过测定病毒性脑炎患者脑脊液中S-100B蛋白及血管内皮生长因子（VEGF）水平，探讨其在病毒性脑炎发病过程中对脑损害的意义。方法选择42例病毒性脑炎患者（病毒性脑炎组）和同期住院治疗的40例其他疾病患者（对照组），分别进行脑脊液常规、生化及细胞学检查，采用双抗体夹心ABC-ELISA法测定脑脊液中S-100B蛋白及VEGF水平，并进行比较。结果病毒性脑炎组脑脊液中自细胞数为（0-584）×10^6/L，对照组为（0-200）×10^6／L。两组脑脊液中蛋白、葡萄糖水平比较差异无统计学意义（P〉0．05），氯化物水平比较差异有统计学意义[（110．10±31．22）mmol／L比（123．80±6．32）mmol／L]（P=0．006）。病毒性脑炎组脑脊液细胞学检查呈混合细胞学反应6例，占14．3％（6／42）。病毒性脑炎组脑脊液中S-100B蛋白水平为25．04-47．97（28．37±6．09）ng／L，对照组为25．0±29．64（26．03±0．90）ng／L，两组比较差异有统计学意义（t=2．462，P=0．018）。病毒性脑炎组脑脊液中VEGF水平为88．84-143．77（96．24±13．38）ng/L，对照组为89．15-96．18（90．67±1．71）ng/L，两组比较差异有统计学意义（t=2．673，P=0．011）。结论病毒性脑炎患者脑脊液中S-100B蛋白及VEGF水平增高均可以支持病毒性脑炎的诊断，动态观察其水平变化对病毒性脑炎患者的病情监测具有重要意义。

[ Abstract ] Objective To investigate the significance of cerebrospinal fluid ~ G5l~＇） ^- lt^l~ protein and vascular endothelial growth factor （VEGF） levels in the pathogenesis of brain injury of viral encephalitis. Methods Forty-two patients with viral encephalitis （viral encephalitis group） and 40 patients with other disease at the corresponding time period （control group） were involved in this study. CSF （routine, biochemistry and cytology ） was detected ,and the levels of S-100B protein and VEGF in CSF were detectedby ABC-ELISA method. Results White blood cell count was （0-584） × 10^6/L in viral encephalitis group, and （0-200）× 10^6/L in control group. The levels of protein and glucose in CSF had no signitieant difference between two groups （P〉 0.05 ）, and the level of chloride in CSF in viral encephalitis group was significantly lower than that in control group [ （ 110.10 ±31.22 ） mmol/L vs. （ 123.80 ± 6.32 ） mmol/L ] （P = 0.006 ）. In viralencephalitis group, cytological examination showed that mixed type cytological reaction was in 6 cases （ 14.3%, 6/42）. The level of S-100B protein in viral encephalitis group [ 25.04-47.97 （28.37 ± 6.09） ng/L ] was significantly higher than that in control group[25.04-29.64（26.03±0.90） ng/L] （t = 2.462,P= 0.018）. The level of VEGF in viral encephalitis group [ 88.84-143.77 （96.24± 13.38 ） ng/L] was significantly higherthan that in control group [89.15-96.18 （90.67 ± 1.71） ng/L]（t =2.673,P=0.011）. Conclusions The high levels of S-IOOB protein and VEGF in CSF could support the viral encephalitis diagnosis. Tracking the levels of S-100B protein and VEGF in CSF dynamically have noticeable effect on checking the condition of viral encephalitis patients.