摘要
以铁皮石斛无菌幼苗为外植体,研究不同基本培养基、生长激素、天然添加物、蔗糖浓度和活性炭浓度对幼苗壮苗的影响。结果表明:苗龄4个月的铁皮石斛幼苗最佳的壮苗培养基为:1/2MS+1.0mg/L NAA+30%椰汁+30g/L蔗糖+2g/L活性炭+8g/L琼脂,pH 5.8。
Taking the seedlings of Dendrobiurn officinale Kimura et Migo as explants, the effects of these factors including minimal medium, growth hormone ,natural additives, sugar and activated carbon on the growth of strengthening plantlet in three months were studied. The results showed that the 1/2MS with 1.0 mg/L NAA, 30% （v/v） coconut juice,30 g/L sugar,2 g/L activated carbon and 8 g/L agar could be used as the best strengthening meadium for D. o f ficinale.
出处
《北方园艺》
CAS
北大核心
2012年第5期132-134,共3页
Northern Horticulture
基金
广西药用植物园青年基金资助项目(桂药基200810)
广西自然科学基金资助项目(桂科自0447040)
关键词
铁皮石斛幼苗
壮苗培养基
椰汁
NAA
Dendrobium officinale Kimura et Migo
strengthening medium
coconut juice
NAA
