天人菊菌核病病原鉴定及其拮抗木霉菌株的筛选

Identification of the Pathogen of Sclerotiniose on Gaillardia pulchella and Screening of Antagonistic Trichoderma Strain

通过致病性测定、病害症状、病原菌培养性状及形态特征等对天人菊菌核病病原菌进行鉴定;通过室内拮抗作用测定和盆栽防治效果测定筛选生防木霉菌株。结果表明:将天人菊菌核病的病原菌鉴定为核盘菌(Sclerotinia sclerotiorum(Lib)de Bary);筛选到对天人菊菌核病菌拮抗作用最强,盆栽防治效果最好的菌株是哈茨木霉H5,其拮抗指数为Ⅰ级,防治效果为93.2%。在测试的5个木霉菌株中,哈茨木霉H5对天人菊菌核病的防治效果最好。

The pathogen was identified based on pathogenicity, disease symptom, cultivation characters and morphology. The antagonistic Trichoderrna strain was screened by antagonistic ability tests in vitro and pot experiments. The results showed that pathogen of sclerotiniose on Gaillardia pulchella was identified as Sclerotinia sclerotiorum （Lib） de Bary. Trichoderma harzianum H5 gave the strongest antagonistic ability in vitro and highest control efficiency in pot experiments. Its antagonistic index and control efficiency were Ⅰ and Trichoderma strains, Trichoderma harzianum H5 had the best control Gaillardia pulchella.