摘要
目的探讨姜黄素联合多烯紫杉醇对人前列腺癌PC-3细胞凋亡的影响及其作用机制。方法将体外培养的PC-3细胞分为对照组(仅加培养基)、姜黄素组(5.0μmol/L姜黄素处理)、多烯紫杉醇组(2.5nmol/L多烯紫杉醇处理)及联合组(5.0μmol/L姜黄素与2.5nmol/L多烯紫杉醇联合处理),作用24h。用流式细胞仪分析PC-3细胞凋亡率,逆转录聚合酶链反应(RT-PCR)和Western blot法分别检测Bcl-2及Bax mRNA和蛋白的表达情况。结果姜黄素和多烯紫杉醇均可诱导PC-3细胞凋亡,二者联合用药组的细胞凋亡率明显高于单药组(P<0.05);RT-PCR及Western blot显示姜黄素和多烯紫杉醇都可明显下调Bcl-2mRNA和蛋白的表达率,而上调Bax mRNA和蛋白表达率,且联合组细胞Bax与Bcl-2表达的改变更明显。结论姜黄素和多烯紫杉醇可能通过下调Bcl-2,上调Bax的表达协同诱导PC-3细胞的凋亡。
Objective To investigate the effects of curcumin combined with docetaxel on apoptosis of human prostate cancer PC-3 cells and its mechanism.Methods Cultured PC-3 cells were divided into control group（only medium added）,curcumin group（treatment with 5.0 μmol/L curcumin）,docetaxel group（treatment with 2.5 nmol/L docetaxel） and combination group（combination treatment of 5.0 μmol/L curcumin and 2.5 nmol/L docetaxel）,and were treated for 24 hours.Flow cytometry was employed to analyze the apoptotic rate of PC-3 cells,reverse transcriptase-polymerase chain reaction（RT-PCR） and Western blot were used to detect the mRNA and protein expression of Bcl-2 and Bax,respectively.Results Both curcumin and docetaxel could induce apoptosis in PC-3 cells,the apoptotic rate of cells in combination group was higher than that in monotreatment groups（P0.05）.RT-PCR and Western blot demonstrated that both curcumin and docetaxel down-regulated mRNA and protein expression of Bcl-2 and up-regulated mRNA and protein expression of Bax,and changes of Bax and Bcl-2 expression in combination group were more significant.Conclusion Curcumin and docetaxel can synergistically induce apoptosis in PC-3 cells via down-regulating Bcl-2 and up-regulating Bax.
出处
《重庆医学》
CAS
CSCD
北大核心
2012年第7期637-639,642,共4页
Chongqing medicine
基金
国家自然科学基金资助项目(30972999)
重庆市自然科学基金资助项目(CSTC2005BB5033)
