VEGF、PCNA及Survivin与膀胱癌病理分级、临床分期的关系

Relationship of VEGF,PCNA,Survivin and pathological grading,clinical stage of bladder cancer

目的探讨膀胱癌肿瘤组织中血管内皮生长因子(VEGF)、增殖细胞核抗原(PCNA)及Survivin的表达及其临床意义。方法采用免疫组织化学链霉素抗生物素蛋白-过氧化物酶(SP)法检测50例膀胱移行细胞癌组织及10例正常膀胱组织中VEGF、PCNA及Survivin蛋白的表达。结果膀胱癌患者肿瘤组织中VEGF、PCNA及Survivin的阳性表达率分别为56.1%(23/41)、56.5%(26/46)和60.0%(27/45),明显高于正常膀胱组织(P<0.01);并随着膀胱癌病理分级和临床分期的增加而升高(P<0.01)。结论 VEGF、PCNA及Survivin的阳性表达与膀胱癌的发生、发展密切相关。

Objective To explore the expression of vascular endothelial growth factor（VEGF）,proliferating cell nuclear antigen（PCNA）and Survivin in bladder tumor tissue and their clinical significance.Methods Immunohistochemistry streptavidin-perosidase（SP） method was employed to detect the expression of VEGF,PCNA and Survivin in tissue of 50 cases of bladder transitional cell carcinoma and 10 cases of normal bladder.Results Positive expression rates of VEGF,PCNA and Survivin in bladder tumor tissue were 56.1%（23/41）,56.5%（26/46） and 60.0%（27/45）,respectively,which were significantly higher than those in normal bladder tissue（P0.01） and increased with increasing pathological grading and clinical stage of bladder tumor（P0.01）.Conclusion Positive expression of VEGF,PCNA and Survivin have close relationship with the bladder tumorigenesis and development.