何首乌提取物二苯乙烯苷对缺氧缺血大鼠星形胶质细胞的影响及其机制的初步探讨

Effects and mechanisms of 2,3,5,4'-tetrahydroxystibene-2-O-β-D-glucoside(TSG) on oxygen-glucose deprived injury of rat astrocytes

目的探讨2,3,5,4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷(TSG)对大鼠大脑皮层星形胶质细胞(AS)缺氧、缺糖损伤的影响,并对其可能的机制进行初步探讨。方法原代培养Sprague-Dawley大鼠大脑皮层AS,建立缺氧、缺糖损伤模型,以四甲基偶氮唑盐(MTT)还原实验检测细胞活力;以乳酸脱氢酶(LDH)漏出率作为细胞损伤指标;以流式细胞术检测细胞凋亡率,研究氧糖剥夺(OGD)对不同组AS的影响。在此基础上,用超氧化物歧化酶(SOD)和丙二醛(MDA)试剂盒测定细胞的氧化应激变化。结果与正常对照组相比,细胞经OGD诱导处理后,细胞存活率降低,脂质过氧化产物MDA含量增加。不同浓度的TSG预处理后能减轻OGD所致的细胞损伤。结论 TSG可抑制OGD诱导的AS损伤,其机制可能与提高SOD活性、降低其消耗率和降低脂质过氧化反应有关。

Objective To investigate the effects of 2,3,5,4＇-tetrahydroxystibene-2-O-^-D-glucoside（TSG） on cerebral cortical astrocytes subiected to oxygen-glucose deprived injury and explore the mechanisms. Methods Primary cultured cerebral cortical astrocytes were prepared from Sprague-Dawley rats, and a cell damage model was induced by oxygen-glucose deprivation （OGD）. The cell damage and viability were evaluated by 3-[4,5-dime- hyl-2-thiazolyl]-2,5-diphenyl-2H-tetrazolium bromide （MTT） reduction assay and lactate dehydrogenase （LDH） released rate. The detection of apoptotic cells was determined by the flow cytometry to study the oxygen-glucose deprived injury on different astrocytes groups. The SOD activity and content of MDA were detected by the kits. Re- sults Compared with the normal control,the cell viability and the activities of antioxidase were decreased,and the MDA contents were increased in OGD-induced group. OGD-induced cell injury was relieved in TSG pretreatment group. Conclusion TSG could inhibit the cell injury induced by OGD in AS,which might correlate with the increas- ing of SOD activity and inhibiting of lipid peroxidation.