肝癌组织Survivin和p-Akt及ER表达及其临床意义分析

Clinical pathology significance of Survivin,p-Akt and estrogen receptor expression in hepatocellular carcinoma tissues

目的:研究原发性肝细胞癌组织和癌旁组织中存活素(Survivin)、磷酸化Akt(p-Akt)和雌激素受体(ER)表达及意义。方法:应用蛋白质印迹法检测52例原发性肝细胞癌和8例正常癌旁组织中Survivin、P-Akt和ER的表达。结果:肝细胞癌组织中Survivin和p-Akt表达明显高于癌旁组织,Survivin和p-Akt的表达与肝癌患者术后复发呈阳性相关(P<0.05),且两者之间的表达也呈阳性相关(r=0.736,P<0.01),而ER在两种组织中表达相仿,与肝癌患者术后复发无相关性,P>0.05。多因素分析结果显示,肿瘤转移、Survivin和p-Akt阳性表达可作为肝癌复发的风险因子。结论:Survivin和p-Akt与原发性肝癌的发生发展关系极为密切,Survivin和p-Akt高表达者预后差,可作为肝癌预后的预测因子。

OBJECTIVE： To investigate the expression of Survivin,phosphorylation of Akt（p-Akt） and estrogen receptor（ER） in hepatocellular carcinoma（HCC） tissues and the relationship between these factors and the prognosis of HCC patients.METHODS： The expression of Survivin,p-Akt and ER was detected by western blot assay in the resected liver specimens from 52 patients with HCC and 8 cases of adjavent normal tissues.RESULTS： The expression of Survivin and p-Akt was positively correlated to the tumor recurrence in HCC patients,respectively（P0.05）,and the positive correlation was also observed between the expressions of Survivin and p-Akt（r=0.736,P0.01）.No correlation was found between ER and the recurrence of HCC patients（P0.05）.Significant differences of disease-free survival rates occurred among subgroups of patients according to the expressions of Survivin and p-Akt.Multivariate analysis revealed that tumor metastasis,positive expression of Survivin and p-AKt were independent risk factors for the recurrence of HCC.CONCLUSIONS： The expression of Survivin and p-AKt in the tissues of HCC closely related to the recurrence of HCC.Survivin and p-AKt may become valuable prognostic factors for HCC patients.