高效液相色谱法同时测定复方扶芳藤合剂中人参皂苷Rb_1和黄芪甲苷的含量

Determination of ginsenoside Rb_1 and astragaloside Ⅳ in compound Fufangteng mixture by HPLC

目的:建立复方扶芳藤合剂中人参皂苷Rb1和黄芪甲苷的含量测定方法。方法:采用HPLC法,色谱柱为Shim-PackCLC-ODS柱(150 mm×6 mm,5μm);流动相为乙睛-水梯度洗脱;流速1.0mL.min-1;柱温为25℃;检测器为PL-ELS2100型蒸发光散射检测器(蒸发温度:115℃,雾化温度:80℃,气体流量:1.1 L·min-1)。结果:人参皂苷Rb1和黄芪甲苷分别在1.39～22.24μg,3.22～51.52μg范围内,其峰面积的自然对数(Y)与进样量的自然对数(X)呈良好的线性关系,r=0.999 5,r=0.999 3;平均加样回收率在97.3%～100.6%之间,RSD在1.1%～2.9%之间(n=9)。结论:该方法稳定、重复性好,结果准确,可作为复方扶芳藤合剂中人参皂苷Rb1和黄芪甲苷的含量测定方法。

OBJECTIVE To establish an RP-HPLC method for the determination of ginsenoside Rb1 and astragaloside Ⅳ in compound Fufangteng mixture. METHODS Chromatographic conditions included Shim-Pack CLC-ODS column （ 150 mm × 6 mm, 5 μm）, the mobile phase consisting of a mixture of acetonitrile-water and a gradient elution was used. The mobile phase flow rate was 1.0 mL. min^-1, column temperature was at 25 ℃, detector： PL-ELS2100 ELSD （ Eva： 115 ℃, Ned： 80℃, gas flow：1. 1 L·min ^-1 ）. RESULTS The linear range of ginsenoside Rbl and astragaloside IV was 1.39-22.24 μg, r = 0. 999 5 and 3.22- 51.52μg, r = 0. 999 3 respectively; The average recovery was between 97. 3%-100. 6 %, and the RSD was between 1.1%-2.9% （n = 9）. CONCLUSION The method is steady and with good repeatability, and can be used to determine the content of ginsenoside Rb1and astragaloside Ⅳ in compound Fufangteng mixture.