载顺铂的纳米羟基磷灰石对A549肺腺癌细胞体外胀亡的影响

Necrosis of The Lung Cancer Cell line A549 Treated with Hydroxyapatite Nanoparticles Carried Cisplatin In Vitro

目的探讨载顺铂的纳米羟基磷灰石对体外培养的人肺腺癌细胞A549增殖及胀亡的影响。方法用机械融合法制备载顺铂的纳米羟基磷灰石粒子,将肺腺癌A549细胞暴露于该粒子中培养。进行形态学观察,MTT法检测载顺铂的纳米羟基磷灰石粒子体外作用于人肺癌A549细胞的量效和时效关系,流式细胞仪分析细胞周期的时相变化。结果载顺铂的纳米羟基磷灰石粒子在体外对人肺癌A549细胞系具有明显的抑制作用,并呈现良好的量效和时效关系。肺癌细胞的生长阻滞于G2/M期,细胞体积增大,胞质空泡化,染色质凝集,胞浆溶解。结论载顺铂的纳米羟基磷灰石粒子具有体外抗肺腺癌系A549的作用,细胞增殖阻滞于G2/M期,阻断细胞周期的进展,导致癌细胞溶解坏死,诱导细胞凋亡是其可能的机制之一。

Objective To explore the influence of the hydroxyapatite （HAP） nanoparticles carried cisplatin on the proliferation and necrosis of the human lung carcinoma cell line A549 in vitro. Methods The hydroxyapatite nanoparticles carried cisplatin was prepared by the method of mechanic combination. The lung cancer cell line A549 was exposed in the nanoparticles in vitro. The morphology change was observed and the time and dose effect-dependent manner were investigated by MTF method. The change of cycle phase was analyzed by FCM. Results The lung cancer cell line A549 was significantly inhibited by hydroxyapatite （HAP） nanoparticles carried cisplatin with good time and dose dependent efficacy. The cell proliferation was arrested at the G2/M phase. Changes such as cell enlarged, cytoplasm vacuoles, chromatin agglutinated and cytoplasm dissolved were observed. Conclusion Hydroxyapatite nanoparticles carried cis-platin have the efficacy of anti-lung cancer cells in vitro. The cell proliferation was arrested at G1 phase and progress of the cell cycle was blocked. These result in necrosis of the tumor cells. It is the possible underlying mechanism that inducing cell apoptosis.