炎性狭窄胆管壁中成纤维细胞表型改变及增殖的意义

A STUDY OF PHENOTYPIC CHANGE AND PROLIFERATION OF FIBROBLASTS IN INFLAMMATORY STRICTURED BILE DUCT WALL

目的 探讨炎性狭窄胆管壁中成纤维细胞表型改变及增殖在炎性胆管狭窄发生中的作用。方法 对人正常胆管壁及炎性狭窄胆管壁组织学结构及其成纤维细胞的密度和超微结构进行观察。结果 成纤维细胞是胆管壁中合成胶原的主要细胞 ;炎性狭窄胆管壁的纤维化增厚明显 ,其成纤维细胞表型改变 ,由相对静止转化为功能活跃状态 ,并进一步向肌成纤维细胞转化 ,且大量增殖。结论 静止型成纤维细胞转化为功能活跃的成纤维细胞及肌成纤维细胞并大量增殖 。

Objective To investigate the phenotypic change and proliferation of fibroblasts in human inflammatory strictured bile duct wall. Methods We observed the density and ultrastructure of fibroblasts, and the histologic structure in human normal bile duct wall and inflammatory strictured bile duct wall by light and electron microscope. Results The results showed that fibroblasts were the main source of extracellular matrix production in bile duct wall. The phenotype of fibroblasts in inflammatory strictured bile duct wall changed obviously, quiescent fibroblasts were activated and transformed to myofibroblasts, with massive proliferation.Conclusion These data suggest that massive proliferation of activated fibroblasts and myofibroblasts is the main source of extracellular matrix overproduction which results in inflammatory bile duct stricture.