睾丸免疫豁免细胞的分离及其功能测定

SEPARATION OF IMMUNE PRIVILEGE TESTICULAR CELL AND EXPRESSION OF FAS LIGAND

目的 探讨睾丸产生免疫豁免的机理。方法 应用胶原酶、胰蛋白酶及脱氧核糖核酸酶消化制备睾丸Sertoli细胞 ,体外与活性淋巴细胞共同培养 ,了解其对淋巴细胞的杀伤作用。并用SABC法标记睾丸组织Sertoli细胞Fas配体的表达。结果 Sertoli细胞可杀伤与之共培养的活性淋巴细胞 ,睾丸组织Sertoli细胞有明显的Fas配体表达。

Objective To investigate the rationale of immune privilege of testicular sertoli cell. Methods Testicular sertoli cell was prepared by digested collagenase, trypsin, and Dnase. In vitro, the sertoli cells were culture together with active lymphocytes to observe the effect on killing lymphocytes. SABC was used for labeling the Fas ligand on testicular sertoli cell.Results In vitro, sertoli cell can kill the active lymphocytes, and testicular sertoli cell expresses the Fas ligand.Conclusion Fas ligand expressing on the testicular sertoli cell may be the cause of immune privilege of testicular.