痹肿消汤及湿热毒淤各拆方对胶原诱导型关节炎大鼠滑膜IL-1和TNF的影响

Effect of bizhongxiao decoction and its decomposed formulas on collagen-induced arthritis in rat synovial effects of IL-1 and TNF

目的从湿热毒淤各方面研究痹肿消汤及拆方对胶原诱导型关节炎模型大鼠滑膜IL-1和TNF的影响,进一步探讨该药物的作用机制。方法 90只健康雌性SD大鼠随机分为正常组、模型组、痹肿消汤组(简称全方组)及清热解毒中药干预组(简称拆方1组)、祛湿中药干预组(简称拆方2组)、活血中药干预组(简称拆方3组)。除正常组之外各组大鼠采用皮下注射牛II型胶原与完全弗氏佐剂建立胶原诱导型关节炎大鼠模型,观察痹肿消汤及拆方对实验性大鼠足肿胀的抑制作用,免疫组化检测不同时间点关节滑膜中炎性细胞因子IL-1和TNF的水平。结果造模21 d后,模型组、痹肿消汤组、拆方1、拆方2组、拆方3组大鼠TNF和IL-1水平明显高于正常组(P<0.05)且模型组TNF和IL-1水平明显高于全方组及拆方1组、拆方2组、拆方3组(P<0.01);随着时间延长,模型组TNF和IL-1水平逐渐升高,痹肿消汤组及各拆方组则逐渐降低。而全方组TNF和IL-1水平低于各拆方组(P<0.05)。其中全方组,拆方1组,拆方2组,拆方3组作用依次减弱。结论痹肿消汤及拆方在胶原诱导型关节炎模型大鼠中可以下调致炎因子IL-1和TNF的水平。

[Objective] To study the effect of bizhongxiao decotion and decompositions on study on IL-1 and TNF-1 of rheumatoid, Arthritis rat, and the mechanism of it. [Methods] 90 healthy normal female SD rats randomly divided into groups, model groups, effect of bizhongxiao decoction unit and heat-clearing and toxicity of traditional Chinese medicine intervention, qushi intervention of traditional Chinese medicine group, activating Chinese herbal medicine intervention group. Apart from the normal group groups using subcutaneous injection of bovine type II collagen in rat established with complete Freund adjuvant in rats with collagen-induced arthritis model, observa- tion on the effect of bizhongxiao decoction and its disassemble formulas on experimental rat inhibition of foot swelling, immunohistochemical detection of various points of inflammatory cytokines in synovial IL-1 and TNF lev- els. [Results ] Modeled after 21 d, model groups, groups and dismantle groups of effect of bizhongxiao decoction in rats significantly higher than narmal levels of TNF and IL-1 （P 〈0.05）. And the model set of TNF and IL-1 level is significantly higher than group effect of bizhongxiao decoction and separated groups （P 〈0.01）. With the extended time, a model set of TNF and IL-1 level rising, effect of bizhongxiao decoction on the Group and the split groups gradually reduced. Effect of bizhongxiao decoction on IL-I and TNF levels below the Group separated groups. All- party group, ingredients 1 group, separated 2 groups, recipes 3 Group in turn weakened. [ Conclusion ] The effect of bizhongxiao decoction and its disassembled prescriptions in rats with collagen-induced arthritis model, you can cut inflammation factor IL-1 and TNF levels.