期刊文献+

影响嗜酸乳杆菌表面疏水性因素的研究 被引量:2

The factors involved in the surface hydrophobicity of Lactobacillus acidophilus
原文传递
导出
摘要 对嗜酸乳杆菌的表面疏水性进行分析,利用微生物黏着碳氢化合物法测定嗜酸乳杆菌的表面疏水率,采用不同条件分别处理菌体细胞表面,初步确定嗜酸乳杆菌表面疏水性的影响因素。经研究得出,影响嗜酸乳杆菌表面疏水性的因素有时间、温度、pH、浓度、Ca2+和蛋白酶,并结合国内外报道得出,嗜酸乳杆菌表面疏水性与黏附能力之间存在着相关性;此外,推测能够介导嗜酸乳杆菌表面疏水性的物质可能是一类蛋白质。 We analyzed the surface hydrophobicity of Lactobacillus acidophilus. We utilized the method of microbial adhesion the hydrocarbons to determine the surface hydrophobic rate of Lactobacillus acidophilus and used the different conditions to process the cell to determine the influencing factors of the surface hydrophobicity of Lactobacillus acidophilus preliminary. Through this research, we obtained that the influencing factors of the surface hydrophobicity of Lactobacillus acidophilus were time, temperature, pH, concentration, Ca^2+ and protease. According to domestic and international articles, we got that the surface hydrophobicity of Lactobacillus acidophilus was connected with the adhesion ability. In addition, we speculated that some substances which could mediate the surface hydrophobicity of Lactobacillus acidophilus may be a class of proteins.
出处 《食品科技》 CAS 北大核心 2012年第3期22-26,共5页 Food Science and Technology
基金 陕西省"13115"项目(2009ZDKG-20) 陕西省教育厅项目(2010JK446)
关键词 嗜酸乳杆菌 疏水性 影响因素 黏附 Lactobacillus acidophilus hydrophobicity influencing factors adhesion
  • 相关文献

参考文献15

  • 1钟燕,黄承钰,何涛,Harmsen HMJ,Vonk RJ.益生菌和酸奶对乳糖不耐受者结肠菌群作用研究[J].卫生研究,2006,35(5):587-591. 被引量:12
  • 2Bernet MF,Brassart D,Neeser J R,et al.Adhesion of human bifidobacterial strains to cultured human intestinal epithelial cells and inhibition of enteropathogen-cell interactions[J].Appl Environ Microbiol,1993,59:4121-4128.
  • 3Candela M,Perna F,Carnevali P,et al.Interaction of probiotic Lactobacillus and Bifidobacterium strains with human intestinal epithelial cells:adhesion properties,competition against enteropathogens and modulation of IL-8production[J].International Journal of Food Microbiology,2008,125:286-292.
  • 4Fooks LJ,Gibson GR.Probiotics as modulators of the gut flora[J].Br J Nutr,2002,88(Suppl1):S39-S49.
  • 5Bengmark S.Use of some pre-,pro-and synbiotics in critically ill patients[J].Best Pract Res Clin Gastroenterol,2003,17:833-848.
  • 6Pan WH,Li PL,Liu Z.The correlation between surface hydrophobicity and adherence of Bifidobacterium strains from centenarians′faeces[J].Anaerobe,2006,12(3):148-152.
  • 7Canzi E,Guglielmetti S,Mora D,et al.Conditions affecting cell surface properties of human intestinal bifidobacteria[J].Antonie Van Leeuwenhoek,2005,88(3-4):207-219.
  • 8林雪彦,牛钟相.乳酸杆菌高黏附性的适宜条件研究[J].中华微生物学和免疫学杂志,2005,25(12):1022-1022. 被引量:4
  • 9马向前,周德庆.双歧杆菌和乳酸菌的一种简便快速计数法[J].微生物学报,1997,37(1):62-64. 被引量:81
  • 10陈罕,樊明文,边专,贺红.变形链球菌表面蛋白不同表达株的疏水性研究[J].口腔医学纵横,1997,13(4):195-197. 被引量:4

二级参考文献47

  • 1钟燕,黄承钰,阴文娅,Vonk RJ.用双标稳定同位素技术分析乳糖酶缺乏者小肠粘膜乳糖酶活性[J].卫生研究,2005,34(3):312-316. 被引量:7
  • 2Collado MC, Meriluoto J, Salminen S. In vitro analysis of probiotic strain combinations to inhibit pathogen adhesion to human intestinal mucus. Food Research International, 2007, 40: 629-636.
  • 3Bernet MF, Brassart D, Neeser JR, Servin AL. Adhesion of human bifidobacterial strains to cultured human intestinal epithelial ceils and inhibition of enteropathogen-cell interactions. Applied Environmental Microbiology, 1993, 59(12) : 4121-4128.
  • 4Candela M, Perna F, Carnevali P, Vitali B, Ciati R, Gionchetti P, Rizzello, Campieri, Brigidi P. Interaction of probiotic Lactobacillus and Bifidobacterium strains with human intestinal epithelial cells: adhesion properties, competition against enteropathogens and modulation of IL-8 production. International Journal of Food Microbiology, 2008, 125: 286-292.
  • 5Kos B, Suskovic J, Vukovic S, . Simpraga M, Frece J, Matosic. Adhesion and aggregation ability of probiotic strain Lactobacillus acidophilus M92. Journal of Applied Microbiology, 2003, 94(6): 981-987.
  • 6Mobili P, Serradell MA., Trejo SA, Puigvert FA, Abraham AG, Antoni GL. Heterogeneity of S-layer proteins from aggregating and non-aggregating Lactobacillus kefir strains. Antonie Van Leeuwenhoek, 2009, 95(4) : 363-372.
  • 7Crociani J, Grill JP, Huppert M, Ballongue J. Adhesion of different bifidobacteria strains to human enterocyte-like Caco-2 cells and comparison with in vivo study. Letters in Applied Microbiology, 1995, 21 (3) : 146-148.
  • 8Del RB, Sgorbati B, Miglioli M, Palenzona D. Adhesion, autoaggregation and hydrophobicity of 13 strains of Bifidobacterium longum. Letters in Applied Microbiology, 2000, 31 (6) : 438-442.
  • 9Palencia PF, Lopez P, Corbi AL, Pelaez C, Requena T. Probiotic strains : survival under simulated gastrointestinal conditions, in vitro adhesion to Caco-2 cells and effect on cytokine secretion. European Food Research Technology, 2008, 227: 1475-1484.
  • 10Buck BL, Altermann E, Svingerud T, Klaenhammer R.Functional analysis of putative adhesion factors in Lactobacillus acidophilus NCFM. Applied Environmental Microbiology, 2005, 71 (12) : 8344-8351.

共引文献118

同被引文献18

引证文献2

二级引证文献6

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部