摘要
研究了培养条件(接种量、发酵温度、初始pH)对LactobacillusJ23发酵合成广谱抗菌肽Bac-J23的影响,并采用响应面法进行了优化。结果表明,Lactobacillus-J23生产Bac-J23体现出温度、pH梯度和接种剂量依赖性,响应面优化结果表明最适温度和pH分别为36.8℃和5.2,接种量阈值为2.13×104cfu/mL。
pH, temperature and inoculation size dependent property were observed when Lactobacillus paracasei-J23 produced Bac-J23. The optimum condition to Bac-J23 synthesize were 36.8 ℃, pHS.2 and 2.13×10^4 cfu/mL inoculum size. Under the optimum condition, the production of Bac-J23 was improved 1.68 folds and reached 5400 U/mL.
出处
《食品科技》
CAS
北大核心
2012年第3期35-39,共5页
Food Science and Technology
基金
国家自然基金项目(30900996)
中央高校基本科研业务费专项资金项目(HIT.NSRIF.201185)
关键词
乳酸菌
抗菌肽
响应面
优化
Lactic acid bacteria
bacteriocin
RSM
optimum
