人参蛋白对小鼠的耐缺氧及抗氧化作用

Anti-hypoxia and anti-oxidant effects of ginseng protein on mice

目的:研究人参蛋白对小鼠耐缺氧及抗氧化能力的影响。方法:通过常压耐缺氧、亚硝酸钠中毒、急性脑缺血性缺氧三项指标评价人参蛋白对小鼠的耐缺氧作用;通过测定肝脏和血清中丙二醛(MDA)含量和谷胱甘肽过氧化物酶(GSH-Px)活性的变化,研究人参蛋白对小鼠体内抗氧化能力的影响。结果:与对照组比较人参蛋白组小鼠常压耐缺氧存活时间、亚硝酸钠中毒存活时间、急性脑缺血性缺氧张口喘气时间均延长;同时人参蛋白能降低肝脏和血清中丙二醛(MDA)的含量,并能提高肝脏和血清中谷胱甘肽过氧化物酶(GSH-Px)的活性。结论:人参蛋白对小鼠耐缺氧能力具有显著增强作用,对D-半乳糖造模小鼠有一定的抗氧化作用。

Purpose： To study the anti-hypoxia and anti-oxidant effects of ginseng protein on mice. Methods： The experiment of anti-hypoxia include three parameters： the survival time of mice in nomal pressure hypoxia surrounding, the survival time of mice inject sodium nitrite and the panting time of mice in acute cerebralischemia, malondialdehyde（MDA） content, glutathione peroxidase（GSH-Px） activity in serum and in liver were assayed to explore the anti-oxidant effect. Results： Compared with the control group, the experimental groups prolong the survival time of mice in nomal pressure hypoxia surrounding, prolong the survival time of mice inject sodium nitrite and the panting time of mice in acute cerebralischemia significantly（P〈0.05）. Results showed that MDA content revealed decrease in serum or liver, while GSH- Px activity revealed increase in serum or liver. Conclusions： The effect of ginseng protein on anti-hypoxia of mice is significant, ginseng protein has anti-oxidant activity in D-galactose-induced senescent mice.