基于香蕉皮水解液的谷胱甘肽生物合成

Fermentative production of glutathione with hydrolyzate of banana peels pretreated by dilute acid

以香蕉皮为原料,研究了可发酵性糖的制备及其用于谷胱甘肽的生物合成,结果发现产朊假丝酵母利用香蕉皮酸解液发酵生产谷胱甘肽是可行的。通过单因素实验和均匀设计法对影响稀硫酸水解香蕉皮粉末的关键因素进行了优化,分别以还原糖得率和谷胱甘肽得率为目标建立了二次多项式数学模型。模型的验证结果表明,最大还原糖得率和谷胱甘肽得率需要不同的酸解条件。在各自最佳的酸解条件下,最大还原糖得率和最大谷胱甘肽得率分别为287.6mg/g和5.40mg/g,该结果为合理利用木质纤维素类生物质原料合成有用化学品的研究提供了借鉴。

The preparation of fermentable sugars from banana peels by dilute acid and its application in the fermentative production of glutathione was investigated in this work, and it is feasible that glutathione can be biosynthesized with Candida utilis using the syrup from banana peel hydrolyzate as the substrate. Single factor experiments and uniform design was applied to optimize the key parameters influencing the hydrolysis of banana peels by dilute sulfuric acid. Second order quadratic equations were established based on the yield of reducing sugars and the yield of glutathione, respectively. The validation of the models showed that the maximum yield of reducing sugars and the maximum yield of glutathione required different reaction conditions for banana peels hydrolysis. Under the maximal conditions for each parameter, the maximum yield of reducing sugars and the maximum yield of glutathione were achieved as 287.6 mg/g and 5.40 mg/g, respectively. The application of banana waste for the production of glutathione will also give a good reference for other useful chemical compounds using lignocellulosic material as substrate.