用SPR生物传感器研究纤维蛋白原在生物医用材料表面的吸附

ADSORPTION OF FIBRINOGEN ON THE SURFACES OF BIOMATERIALS STUDIED BY USING SURFACE PLASMON RESONANCE (SPR) SENSOR

材料表面对血浆蛋白的吸附特性 ,是研究和评价生物医用材料血液相容性的重要依据。本文用自制的表面等离激元 (SPR)传感器 ,测量了金膜、磷脂DSPC膜、成都科大Ⅱ型聚氨酯、Pellethane2 36 3 55D聚氨酯及有机玻璃膜表面对纤维蛋白原的动态吸附特性 ,在纤维蛋白原溶液浓度为5mg/ml的相同条件下 ,磷脂DSPC膜表面吸附纤维蛋白原的速度最低 ,饱和吸附浓度也最小 (表面浓度为 1ng/mm2 )。其次是裸金膜 (表面浓度为 3.5ng/mm2 ) ,再其次是成都科大Ⅱ型聚氨酯膜 (表面浓度为 3.8ng/mm2 )和Pellethane 2 36 3 55D聚氨酯 (表面浓度为 4 .3ng/mm2 ) ,吸附速度和吸附量最高的是有机玻璃膜 (表面浓度为 4 .5ng/mm2 )。结果表明 ,材料表面对纤维蛋白原的吸附动力学特性 ,与材料的血液相容性密切相关。表面等离激元技术与本文采用的在金膜上铺展高分子材料的离心铺膜法和LB技术等样品制备技术相结合 ,为生物材料表面对蛋白质吸附特性的实时、动态、原位研究提供了一种新的高灵敏度的方法 ,并可能发展成为一种材料生物相容性的测试和评价的新方法。

In this work, dynamic characteristics of fibrinogen adsorption onto the surfaces of gold, monolayer of distearoyl phosphatidylcholine (DSPC), polyurethane(PU) made by Chengdu University of Science and Technology (CUST TypeⅡ PU),PU made by DOW Chemistry, USA (Pellethane 2363 55D) and plexiglass were measured by using a self made surface plasmon resonance (SPR) sensor. Different behavior of fibrinogen adsorption onto different surfaces were observed at the same fibrinogen concentration of 5 mg/ml. The phospholipid monolayer showed the lowest speed and the smallest equilibrium surface concentration of adsorbed fibrinogen (C s=1ng/mm 2),followed by the gold film (C s=3.5ng/mm 2),CUST TypeⅡ PU(C s=3.8ng/mm 2) and pellethane 2363 55D(C s=4.3ng/mm 2). The plexiglass has shown the highest speed and the largest equilibrium surface concentration of adsorbed fibrinogen (C s=4.5ng/mm 2). It showed that the dynamic characteristic of fibrinogen adsorption onto material surface was closely related to the hemocompatibility. SPR technique combined with the methods of material spreading onto the surface of gold developed in this work, including the polymer spreading by centrifugation and lipid spreading by LB technique, provided us a very sensitive tool for the real time, dynamic and in situ study of protein adsorption on the surface of biomaterials. It will be promising to use these techniques in the measurement and evaluation of biocompatibility of materials.