高效液相色谱法测定糖汁中没食子酸含量的研究

HPLC determination of gallic acid in cane sugar juice

建立准确测定甘蔗糖汁酚类色素没食子酸含量的高效液相色谱法。采用Hypersil BDSC18柱(4.6mm×250mm,5μm),流动相为乙腈-0.05%磷酸(5:95),检测波长是280nm,流动相流速为1.0mL/min。没食子酸线性范围为20～100mg/L,回归方程Y=15960X-4749,相关系数0.9991,检出限0.22mg/L,平均加标回收率为101.45%(RSD为1.13%)。样品清汁、清糖浆配成浓度15°Bx后,过0.45微滤膜,测定滤液中没食子酸含量,分别是21.70、17.06mg/L。结论:高效液相色谱测定方法准确、可靠,可用于甘蔗糖汁色素中没食子酸的检测。

Aim To establish a method for the detection of gallic acid in cane sugar juice. Methods： HPLC was on a Symmetry C18 analytical colume（4.6 mm×250 mm, 5 μm） with acetonitrile-0.5% phosphoric acid and flow rate was 1.0 mL/min. The UV detection wavelength was set at 280nm and column temperature was 25 %. Results： The linear range of gallic acid was 20-100 mg/L, the regression equation was Y=15960X-4749 （r=0.9991）, with a detection limit of 0.22 mg/L The recover of measure in analyzing of gallic was 101.45%（RSD=1.13%）. The clarified sugar juice and syrup were prepared into the concentration of 15°Bx, and then filtrated by 0.45 micro-filtration membrane, the content of gallic acid were 21.70 mg/L, 17.06 mg/L respectively. Conclusions： The HPLC method was accurate and reliable in analyzing the gallic acid of cane sugar juice.