桃儿七中黄酮类成分的分离与鉴定

Isolation and identification of flavonoids from the roots and rhizomes of Sinopodophyllum emodi

目的对桃儿七的95%(φ)乙醇提取物的化学成分进行研究。方法利用反复硅胶、SephadexLH-20、开放ODS柱色谱等方法进行分离纯化;根据理化性质及波谱分析对分离得到的化合物进行结构鉴定。结果分离得到8个黄酮类化合物,分别鉴定为山柰酚(kaempferol,1)、槲皮素(querce-tin,2)、杨梅素-3',4'-二甲醚(myricetin-3',4'-dimethyl ether,3)、3-O-甲基-槲皮素(3-O-methyl-quercetin,4)、山柰酚-3-O-β-D-葡萄糖苷(kaempferol-3-O-β-D-glucopyranoside,5)、槲皮素-3-O-β-D-葡萄糖苷(quercetin-3-O-β-D-glucopyranoside,6)、芦丁(rutin,7)、山柰酚-3-O-芸香糖苷(kaempfer-ol-3-O-rutinoside,8)。结论化合物3、4、8为首次从该属植物中分离得到。

Objective To isolate and identify the chemical constituents from the 95% （φ） EtOH extract of the roots and rhizomes of Sinopodophyllum emodi. Methods Compounds were isolated by various kinds of column chromatography on silica gel, Sephadex LH-20 and ODS, and their structures were elucidated by the physico-chemical characters and the spectral analysis. Results Eight flavonoids were obtained and identified as kaempferol （ 1 ）, quercetin （2）, myricetin-3 ＇, 4＇-dimethyl ether （ 3 ）, 3-O-methyl-quercetin （ 4 ）, kaempferol- 3-O-β-D-glucopyranoside （ 5 ）, quercetin-3-O-fl-D-glucopyranoside （ 6 ）, rutin （ 7 ）, and kaempferol-3-O-rutinoside（8 ）, respectively. Conclusions Compounds 3,4 and 8 are isolated from Sinopodophyllum genus for the first time.