RP-HPLC法测定清胰利胆颗粒中绿原酸和芍药苷的含量

Determination of chlorogenic acid and paeoniflorin in Qingyilidan granules by RP-HPLC

目的建立同时测定清胰利胆颗粒中绿原酸和芍药苷含量的方法。方法采用HPLC法。色谱柱:Kromasil C18柱(250 mm×4.6 mm,5μm),流动相:乙腈(A)-体积分数为0.1%的磷酸水溶液(B),梯度洗脱程序:0 min-10%(φ)A、9 min-13%(φ)A、13 min-13%(φ)A、14 min-16%(φ)A、22min-17%(φ)A、30 min-17%(φ)A、40 min,40%(φ)A,流速:1.0 mL.min-1,检测波长:327 nm(0～14 min)和230 nm(14～40 min)。结果绿原酸和芍药苷质量浓度分别在5.2～51.6 mg.L-1(r=0.999 6,n=6)、9.9～99.2 mg.L-1(r=0.999 8,n=6)内与峰面积线性关系良好,平均回收率分别为100.5%(RSD=1.9%,n=6)、100.2%(RSD=2.2%,n=6)。结论该方法可作为清胰利胆颗粒质量控制方法之一。

Objective To develop an HPLC method for simultaneous determination of chlorogenic acid and paeoniflorin in Qingyilidan granules （traditional Chinese medicines）. Methods Analyses were carried out on Kromasil C18 column （ 250 mm × 4. 6 mm, 5 μm）. The mobile phase was acetonitril （A） -1% （φ） acetic acid （B） with gradient elution （0 min, 10% （φ） A; 9 rain, 13 % （φ） A; 13 min, 13 % （φ） A; 14 min, 16% （φ） A; 22 rain,17% （φ） A;30 rain,17% （φ）A;40 min,40% （φ）A and the flow rate was 1.0 mL.min-1 ,the detection wavelength was set at 327 nm（0- 14 min）and 230 nm（ 14-40 min）. Results The linear calibration curve were obtained in the concentration range of 0. 676-10. 14 mg. L-1（ r = 0. 999 6, n = 5 ） for chlorogenic acid and 0. 276-3.680（ r =0.999 8 ,n=5） for paeoniflorin. The mean recoveries of chlorogenic acid arid paeoniflorin were 96. 7% （ RSD = 1.7%, n = 6 ） and 96. 4% （ RSD = 1.0%, n = 6 ）, respectively. Conclusions The method can be used to control the quality of Qingyilidan granules, which provides the advantage of quickness, simplicity and repeatability.