槟榔药材C_(18)柱化学特征图谱的研究

Study on the Characteristics of Betel Nut on C_(18) Column

目的:建立槟榔对照药材的C_(18)柱化学特征图谱和评价指标。方法:以Inertsi1ODS-3 C_(18)(250 mm×4.6 mm,5μm)-为色谱柱;以甲醇-0.15%三乙胺溶液(磷酸调pH至6.5)(13:87)为流动相,检测波长为215 nm。结果:供试品特征图谱中呈现3个特征峰,与参照物峰相应的峰为S峰,计算各特征峰与S峰的相对保留时间。相对保留时间规定值:1.10(峰2)、1.63(峰3)。结论:14批样品的相似度均在0.9以上,且方法学考察的结果均符合指纹图谱技术要求。

Objective： To establish specific analysis methods and evaluation index of betel nut by HPLC on C18 column. Method： The determination was carried out on an Inertsil ODS-3 C18 （250 mm ×4.6 mm,5 μm）column using methanol- 0. 15% triethylamine solution （adjusting pH value to 6.5 with phosphoric acid） （ 13：87 ） as the mobile phase, and the detection wavelength was set at 205 nm. Result： There were 3 characteristic peaks on the specific ehromatograms and the S peak was considered as the reference. Compared with the S peak, the relative retention time of the other two characteristic peaks was 1.10 （ peak 2） and 1.63 （ peak 3 ）, respectively. Conclusion ： The similarity degree of 14 batches specific ehromatograms is above 0. 9, and the validations of the methods all meet the technical requirements of fingerprint.