HPLC法检测人血浆中百草枯的浓度

Determination of Paraquat in Human Plasma by HPLC

目的:建立检测人血浆百草枯浓度的高效液相色谱方法。方法:流动相为乙腈-3 mmol·L^(-1) SDS-0.2%TFA(35:28:37),流速为1.0 ml·min^(-1),检测波长258 nm,柱温35℃,内标为卡马西平。用乙腈沉淀血浆蛋白处理后,进行高效液相色谱分析。结果:血浆百草枯在0.5～100.0 mg·L^(-1)范围内线性关系良好(r=0.999 8)。高、中、低三个浓度(1.00,10.00,50.00 mg·L^(-1))的相对回收率分别为(103.06±4.04)%,(100.20±3.00)%和(99.33±2.26)%;日内RSD分别为4.85%,5.97%和4.91%;日间RSD分别为6.60%,5.98%和4.10%。结论:本方法准确可靠,简便快速,适用于百草枯中毒患者的血药浓度检测。

Objective： To develop an HPLC method for the determination of paraquat in human plasma. Method： The mobile phase was acetonitrile-3 mmol .L^-1 SDS-0.2%TFA （35：28：37） with the flow rate of 1.0 ml ·min^-1, the detection wavelength was 258 nm, the column temperature was 35℃ and the internal standard was carbamazepine. Result： Excellent linear relationship was ob- tained within the range of 0.50 mg ·L^-1 to 100.00 mg·L^-1 （r =0.999 8）. The relative recovery was （103.06 ＋4.04）%, （100.20 ＋ 3.00） % and （99.33 ＋ 2.26） % for three concentrations, respectively. The intra-day RSD was 4.85%, 5.97 % and 4.91%, and inter-day RSD was 6.60%, 5.98% and 4.10%, respectively. Conclusion： The method is accurate, simple and rapid, and can be used to determine the human plasma concentration of paraquat.