摘要
The growth hormone gene (GH) affects animal growth and is a potential target for genetic studies of variation related to growth traits. In this study, we analyzed single nucleotide polymorphisms (SNPs) in GH intron regions and their associations with growth traits in large yellow croaker, Larimichthys crocea, from Zhejiang and Fujian stocks. The results of PCR-single strand conformation polymorphism showed two haplotypes of intron 1, named AA and AB genotypes, in Zhejiang stock. AB exhibited an SNP at position 196 (G A) that was negatively correlated with body height and positively correlated with standard length/body height (P 0.05). Two different genotypes, CC and CD, were identified in intron 2 in Fujian stock, with CD showing an SNP at position 692 (T C). The CD genotype had a significantly positive correlation with both weight and total length (P 0.01). These basic data highlight the potential for using GH as a genetic marker of fish growth in marker assisted selection.
The growth hormone gene （GH） affects animal growth and is a potential target for genetic studies of variation related to growth traits. In this study, we analyzed single nucleotide polymorphisms （SNPs） in GH intron regions and their associations with growth traits in large yellow croaker, Larimichthys crocea, from Zhejiang and Fujian stocks. The results of PCR-single strand conformation polymorphism showed two haplotypes of intron 1, named AA and AB genotypes, in Zhejiang stock. AB exhibited an SNP at position 196 （G→A） that was negatively correlated with body height and positively correlated with standard length/body height （P≤0.05）. Two different genotypes, CC and CD, were identified in intron 2 in Fujian stock, with CD showing an SNP at position 692 （T→C）. The CD genotype had a significantly positive correlation with both weight and total length （P≤0.01）. These basic data highlight the potential for using GH as a genetic marker of fish growth in marker assisted selection.
基金
Supported by the National Basic Research Program of China(973Program)(No.2010CB126304)
the Special Fund for Agro-scientific Research in the Public Interest(No.200903046)
the Project from the Ministry of Science and Technology of China(No.2006DKA30470017)
关键词
生长激素基因
单核苷酸多态性
生长性状
内含子
大黄鱼
单链构象多态性
聚合酶链反应
标记辅助选择
large yellow croaker （Larimichthys crocea）
growth hormone gene （GH）
PCR-single strandconformation polymorphism （PCR-SSCP）
single nucleotide polymorphism （SNP）
growth trait
