脑出血后胰岛素样生长因子-1表达与中风闭、脱证关系的研究

Study on relationship among expression of IGF1 in perihematomal brain region,excessive and depletion syndrome of stroke after cerebral hemorrhage

目的观察脑出血（intracerebral hemorrhage，ICH）患者血肿周围脑组织中胰岛素样生长因子．1（IGF．1）的表达与中风闭、脱证的关系。方法28例脑出血患者根据辨证分为3组：阴闭组、阳闭组、脱证组，另选6例非正常死亡者3h内获取的正常脑组织标本，采用免疫印迹法检测血肿边缘区IGF．1的表达，研究IGF．1的表达与中医闭、脱证的关系。结果ICH患者血肿周围脑组织中IGF．1表达明显高于正常对照组（P=0．00），阳闭、阴闭、脱证三组之间血肿周围组织IGF-1表达[分别为（0．696±0．111）、（1．151±0．185）、（1．697±0．108）]两两比较，差异均有统计学意义（P〈0．01）。结论脑出血后脑组织中IGF．1表达量的高低可反映病情轻重。

Objective To observe the expression of IGF-1 in the perihematomal brain region of Intracerebral Hemorrhage（ICH） patients, and to investigate the relationship among IGF-1, excessive and depletion syndrome of stroke. Methods 28 patients were evenly and randomIy recruuted into 3groups： Yin excessive group, Yang excessive group and depletion group. Additional 6 persons of normal death within 3 hours were taken their brain tissue. The IGF-1 expression was detected with Western blotting method.The correlation among the expression of IGF-1, excessive and depletion syndromes were observed. Results The expression of IGF-1 in the pcrihematomal brain region of ICH patients significantly increased in comparison with the normal control group （P=0.00）. The expression of IGF-1 was significantly different among Yang excessive, Yin excessive and the depletion syndromes [ （0.696±0.111）, （1.151±0.185）, （1.697±0.108）, P〈0.01], with the expression of IGF- 1 of depletion syndrome ranked the highest, followed by Yin excessive and Yang excessive syndrome. Conclusion The expression of IGF-1 could reflect the degree of brain impairment.