伊乐藻-固定化氮循环菌技术入湖河道修复研究

Study of Elodea nuttallii-immobilized nitrogen cycling bacteria restoration in an inflow river channel of water source,Taihu Lake

从太湖金墅湾水体筛选出包括土著氨化、亚硝化、硝化和反硝化细菌的氮循环菌，固定于多孔性载体内，对伊乐藻一固定化氮循环菌联用技术在秋冬季太湖金墅湾水源地入湖河道水体生态修复效果进行了研究,经室内生态修复模拟与原位围隔实验表明，伊乐藻-固定化氮循环菌联用对水质改善效果要优于单独使用伊乐藻或固定化氮循环菌，该技术对原位入湖河道有效去除率为：总氮5．9％-1．2％，氨氮12．4-70．3％，硝氮6．1％-68．O％，COD4．2％-78．5％；通过氮循环菌释放可明显提高水体氮循环菌数量，MPN值比对照水体高出3-4个数量级；相关性分析表明，差异性显著（P〈O．01）．经5个月原位围隔试验表明，伊乐藻-氮循环菌联用技术可有效降低秋冬季入湖河道营养盐负荷，有助于控制湖泊水源地富营养化。

Nitrogen cycling bacteria, including ammonifying bacteria, nitrobacteria, nitrosobacteria and denitrifying bacteria were screened and immobilized to the porous carries from nature water of Jinshu Bay, Taihu Lake. The restoration effect of Elodea nuttallii-Immobilized Nitrogen Cycling Bacteria （INCB） assemblage technology applied in one of the inflow river channels of Jinshu bay water source, Taihu Lake was studied in autumn and winter. Lab ecological restoration simulation and in situ enclosure experiment demonstrated that Elodea nuttallii-INCB assemblage technology has better water quality improvement ability than either use INCB or Elodea nuttallii separately. We applied this technology in the inflow river channel water restoration and TN concentration reduced 5.9%-61.2%, NO3 concentration reduced 6.1%-68.0% and COD concentration reduced 4.2%-78.5% during the experiment process. The result also showed nitrogen bacteria quantities increased significantly after releasing INCB, and the MPN values of denitrifying bacteria in the enclosure was 3-4 order of magnitudes higher than the comparison water. There is significant difference between them （P〈0.01）. In situ enclosure experiment lasted for five months showed Elodea nuttallii-INCB assemblage technique can efficiently decrease inflow river nutrient loading and is helpful in water source eutrophication control in autumn and winter.