摘要
球形芽孢杆菌BS-10(Bacillus Sphaericus-10)是蚊幼虫的一种病原体,对库蚊等蚊幼虫具有极高的毒杀作用。1982年从江苏扬州市郊区孳生地分离到,经鉴定属血清5_a5b型,其生理生化特性与世界卫生组织(WHO)推荐的1593、2362菌株相似,但其毒力比1593菌株高1.78—3.2倍,比2362的发酵毒力提高1.79倍。BS-10的杀蚊毒素蛋白的分子量为45KD,主要分布于孢子囊、芽孢壁内,并有菱形的伴孢晶体毒素。当蚊幼吞食一定数量的芽孢后即中毒死亡,吞食数与死亡速度成正相关。最快6h可毒杀,但蚊幼的龄期越小越敏感。它的杀蚊谱为:淡色库蚊>致乏库蚊>凶小库蚊>三带喙库蚊>中华按蚊>埃及伊蚊。BS-10灭幼剂使用量为每m^2投药3～5ml,24h蚊幼死亡率为80％左右,48h为95～100％;随着用药量增加,持效期延长,一般为15～25天。BS-10对人畜、禽及水生生物安全无毒,不污染环境。
Bacillus Sphaericus-10 (BS-10) is a pathogenic bacterium on the larvae of mosquito.It can kill the Culex pipiens pallens Coquillett highly. The serrtype H5a5b was classifledfrom the surburb, Yangzhou in 1982. It is similar to the strains 1593, 2362 recomended by WHO in the characteric of physiology and biochemistry But its toxicity is 2.78—4.2 times than the strain 1593 2.78 times more than the strain 2362. The molecular weight of toxin protein of BS 10 is 45KD), the proteinmainly exists in the sporange andspore cell of BS-10 with a square parasporal cristaline. When the larve of mosquito ate some spores, at most, 6 hr. later it would die, and the death is right proportion to the quantity of diet, and the younger the more sensltive. Its control spectrumas follows: Culex pipiens Coquillett C. pipiens quinuefascitatus Say C.trilaencorhynchus Giles Anopheles sin ensis Wiedean Ae. aegypti Linnaeus. Usage of BS-10 is 3-5ml/m^2, 80％ of the death after 24 hr.; 95-100％ after 48 hr., generaly 15-25Days of persistence. What's more, the product of BS-10 is no harm to the human, beast, poultry, and aquaticlife, and no pollution of environment. Key words: Bacillus sphaericus-10 larvae of mosquito BioIogical characteristic Product of BS-10
