血管内皮生长因子、基质金属蛋白酶-2、-9在舌鳞状细胞癌组织中的表达及其临床意义

The expression and clinical significance of MMP-2 、MMP-9 and VEGF in tongue squamous cell carcinoma

目的探讨血管内皮生长因子（VEGF）、基质金属蛋白酶-2（MMP-2）及基质金属蛋白酶-9（MMP-9）在舌鳞状细胞癌组织中的表达及其临床意义。方法采用免疫组织化学sP法检测60例舌鳞状细胞癌及20例正常舌组织手术切除标本中VEGF、MMP4与MMPO的表达。结果舌鳞状细胞癌组织中VEGF、MMP4与MMPO的表达明显高于正常舌组织（均P〈0．05），Ⅲ＋Ⅳ期和有淋巴结转移的患者又明显高于Ⅰ＋Ⅱ期和无淋巴结转移的患者（均P〈0．05）。舌鳞状细胞癌VEGF和MMP-2与MMPO的表达呈正相关（r=0．489、0．512，P〈0．05）。结论VEGF、MMP-2与MMPO在舌鳞状细胞癌中的表达与淋巴结转移、TNM分期密切相关，VEGF、MMP4与MMPO在舌鳞状细胞癌生长、侵袭和转移过程中可能起着重要的作用，并具有协同作用。

Objective To investigate the expression of vascular endothelial growth factor （VEGF）, matrix metalloproteinase-2（MMP-2） and matrix metalloproteinase-9 （MMP-9） in tongue squamous cell carcinoma and the clinical significance. Methods The expression of MMP-2, MMP-9 and VEGF in 60 cases of tongues quamous cell carcinoma tissues and in 20 cases of normal tongues tissues were examined by immunochemistry. Results The expression of MMP-2, MMP-9 and VEGF was higher in tongues quamous cell carcinoma than that in normal pancreatic parenchyma. In cases with lymph nodes metastasis and TNM stage Ⅲ＋Ⅳ, the expression of two markers were significantly higher than those without lymph nodes metastasis and TNM stage Ⅰ＋Ⅱ （P 〈 0.05）. A positive correlation was found between the expression of VEGF and MMP-2, MMP-9 in tongues quamous cell carcinoma （ P 〈 0. 05 ）. Conclusion The expression of MMP-2, MMP-9 and VEGF was closely correlated with lymph nodes metastasis and TNM stage of tongues quamous cell carcinoma（P 〈0.05）. VEGF and MMP-2, MMP-9 play an important role in the development of tongues quamous cell carcinoma, may cooperate with each other.