摘要
应用抗EHFV单克隆抗体和兔抗EHFV抗体分别致敏醛化血球,建立了检测EHF抗原的反向间接血凝法。检测18株EHF毒株(人源6株、鼠源10株、螨源2株)的细胞培养上清液,以及10份EHF抗原阳性的小白鼠乳鼠的20％的脑悬液,血凝试验均为阳性,最高效价达1:1024,10份正常细胞培养上清液和10份正常小白鼠乳鼠的脑悬液均为阴性。且EHF抗原阳性的标本可被特异的EHF抗体所阻断。本法具有特异、敏感、快速、简便等优点,将有助于临床EHF病人早期诊断方法的建立。
This paper reports the results of experiments of RPHA for the diagnosis of EHF-Ant/gen in 18 samples of supernatent of cell culture and 10 samples of brain suspension of suckllng mice. Ali samoles which contained EHF-Antigen were positive but the controls are negative, and the titer could reach 1: 1024. The samples which were positive of EHF-- Antigen could be blocked specially by the antibody of EHFV. This method employed the aldehyde sheep red blood cells and high titer EHF-Antiebdy of rabbit serum or McAb thus it has ma^y advantages such as Spcioficity sensitivity simplicity and rapidity.
