摘要
本文报告应用间接ELISA法检测60份经IFAT检测确诊的EHF病人血清,结果全部阳性;检测21份临床诊断的病人血清(IFAT均阴性),有5份阳性(23.8％);检测17份其它病人血清和40份正常人血清均为阴性。经ELISA抑制和阻断试验证实本法是特异的,比IFAT法有更高的敏感性,可推广应用于流行病学监测和临床诊断。
Using infected Vero-E6 cell antigen as coating antigen we established an indirect ELISA method for detection of the IgG antibody against EHF virus. The specificity of this method was confirmed by blocking and inhibition tests. It was found to be much more sensitive compared with IFA. This method would be usefull for epidemilologic surveillance and clinical diagnosis.
