组合抗性筛选法选育达托霉素高产菌株

Screening of high daptomycin-producing strains by combined antibiotics resistance screening method

目的利用组合抗性筛选法选育达托霉素高产菌株。方法以玫瑰孢链霉菌(Streptomyces roseosporus ATCC 11379)为出发菌株,通过在不同浓度梯度的达托霉素和链霉素复合抗性平板上进行抗性筛选。结果筛选到一株高产达托霉素的突变株D1000-S3-2,经摇瓶发酵验证达托霉素发酵单位可达59mg/L,比出发菌株提高了63.8%。结论实验证明组合抗性筛选是一种简单高效筛选方法。

Objective Combined antibiotics resistance screening method was applied to screen daptomycin high-producing strain. Methods Based on the biosynthetic pathway and metabolization mechanism of daptoomycin, combined antibiotics resistance screening method was applied to screen daptomycin high-producing strain from original strain of Streptomyces roseosporus ATCC 11379. Results A mutant strain D1000-S3-2 was obtained. The yield of daptomycin was 59 mg/L in flask by the mutant strain, which increased 63.8%, compared to that of the parental strain. Conclusion It was demonstrated that the method provides a fast and effective way of screening S. roseosporus.