狗皮膏大鼠长期毒性试验的体内血液铅变化研究

Study on blood lead of rats in long-term toxicity test with Goupi Gao

目的:建立火焰原子吸收光谱法测定血铅方法,测定狗皮膏长期毒性试验的大鼠血铅浓度动态变化规律,为狗皮膏的安全性用药提供参考。方法:连续外用给予大鼠高、中、低剂量(7,3.5,1.75 g.kg-1)狗皮膏90 d,于给药前、给药后10,30,45,52,60,90 d及停药后16,28 d取血,采用微波消解仪消解血液样品,用石墨炉原子吸收光谱法测量血铅含量。结果:经方法学考察本次试验的标准曲线回归方程A=0.004 9X+0.017,r=0.999 5,检测限为0.380μg.L-1,精密度检查RSD 1.4%,混合血样中血铅值175.77μg.L-1,RSD 6.0%。大鼠给予狗皮膏后,血铅浓度逐渐升高,给药中、低剂量30 d后及高剂量10 d后血铅浓度达到稳定,给药量与血铅浓度具有剂量依赖性,停药后血铅浓度降低。结论:本方法测定准确可靠,适合于血铅的测定,狗皮膏长期大剂量外用可引起血铅升高。

Objective： To observe dynamic changes of blood lead concentration in rats with long-term toxicity test with Goapi Gao by the flame atomic absorption spectrometry, in order to provide reference for safe administration of Goupi Gao. Method： The rats were administered with Goupi Gao by high-dose （7 g . kg-1 ） , medium-dose （3.5 g . kg-1 ） , low-dose （ 1.75 g . kg-1 ） by external use for consecutively 90 days. Then, the blood samples were collected from the rats before the administration and at 10, 30, 45, 52, 60, 90 d after the administration respectively, as well as 16 d and 28 d after the drug withdrawal. The samples were dispelled with microwave digestion system and then were determined by graphite furnace atomic absorption spectrometry for blood lead levels. Result： According to methodological study, the standard curve regression equation in this experiment was A = 0. 004 9X ＋ 0. 017, r = 0. 999 5, with the detection limit up to 0. 380 ug. L-1. The RSD was 1.4% by precision checks. Blood lead level of mixed blood samples was 175.77 ug . L- 1 , whose RSD was 6.0%. Blood lead concentration gradually increased after low-dose and medium-dose administration to rats and became stable at the 10th day and the 30th day by high-dose. Dose is directly related to blood lead concentration. Meanwhile, the blood lead concentration decreases after the drug withdrawal. Conclusion ： The method of determination in this experiment is so accurate and reliable that it can be used for the determination of blood lead. Long-term and high-dose external use of Goupi Gao can increase blood lead.