朱红膏对破损皮肤模型大鼠汞蓄积及肾组织形态的影响

Effects of Zhuhong ointment on mercury cumulation and renal organization modality in skin-impaired model rat

目的:通过对破损皮肤模型大鼠连续给予朱红膏4周和停药4周各相关指标的检测,探讨其对汞蓄积和肾组织形态的影响,为临床安全用药提供科学依据。方法:将大鼠随机分成4组:破损皮肤对照组、朱红膏低、中、高剂量组。于连续给药4周和停药4周后,分别测定尿汞、血汞、肾汞、β-N-乙酰氨基葡萄糖苷酶(NAG)、β2-微球蛋白(β2-MG)、血清尿素氮(BUN)、肌酐(SCr)、尿蛋白(PRO)和尿液pH,计算肾脏系数,进行肾脏组织形态学观察。结果:连续给药4周,3个剂量组大鼠尿汞、血汞和肾汞含量与对照组比较显著升高(P<0.01),且存在量毒关系。停药4周,3个剂量组大鼠尿汞和血汞含量均恢复正常,肾汞含量仍明显升高(P<0.01)。高剂量组大鼠NAG显著升高。停药4周,不同剂量组与对照组比较没有差异。结论:对破损皮肤大鼠长期大剂量使用朱红膏会出现汞蓄积,有可能造成肾组织损伤,临床使用朱红膏应积极监测患者体内的汞含量和肾功能指标。

Objective： To study the effects of Zhuhong ointment on accumulation in the body of mercury and the pathological morphology changes of kidney, via the measurement of related indicators of the skin-impaired model rat. Method ： Eighty-eight SD rats were randomly divided into the impairment control group, and high-, middle-, low-dose Zhuhong ointment groups. Each group was treated by corresponding methods for 4 weeks, and recovering for 4 weeks. Urinary potein （PRO） , pH, Beta N-acetyl aminoglycosidase enzymes （NAG） and β2-microglobulin （β2-MG） contents in urine were taken as monitoring indexes, blood urea nitrogen （BUN） and serum creatinine （SCr） in blood and the levels of mercury in urine, blood and kidney were tested, and the pathological morphology changes of kidney were observed. Result ： After treatment for 4 weeks, compared with impairment control group, the levels of mercury in urine, blood and kidney in every dose group increased significantly （P 〈 0. 01 ）. And the relation exists between toxicity and dose on Zhuhong ointment. After recovery for 4 weeks, the levels of mercury in urine and blood in every dose group restore normal, while the level of mercury in kidney in high- dose group still increased （P 〈0. 01 ）. The level of NAG increased only in high- dose group. There was no significant difference in NAG contents between Zhuhong ointment groups and the impairment control group （ P 〈 0. 05 ）. Con- clusion： Excess using Zhuhong ointment repeatedly may lead to accumulation of mercury and pathological morphology changes of kid- ney. So the levels of mercury in the body and related indicators of renal functions should be tested in clinical when long-term using Zhuhong ointment.