在线液相色谱法和液相色谱-电喷雾质谱联用法检测桑枝中抗氧化活性成分

Detection of antioxidant active compounds in Mori Ramulus by HPLC-MS-DPPH

目的:研究桑枝中的主要抗氧化活性物质。方法:采用自由基在线液相色谱法和液相色谱-电喷雾质谱联用法,鉴定桑枝中主要抗氧化活性成分,然后采用高效液相色谱-DAD(检测波长320 nm)对其含量进行定量分析。结果:桑枝乙醇提取液中的主要抗氧化活性物质确定为氧化芪三酚。高效液相色谱-DAD定量测定桑枝氧化芪三酚,在14～1 260 mg.L-1具有良好的线性关系(r=1.000),平均加样回收率为98.2%,RSD 1.2%。方法准确灵敏、快速简便。桑枝中富含氧化芪三酚,其含量在品种间存在显著差异。结论:自由基在线液相色谱法结合液相色谱-质谱联用法可定性定量分析桑枝中的抗氧化活性物质。

Objective： To study major autioxidant active compounds in Moil Ramulus. Method： The combination of on-line HPLC method and liquid chromatography-mass spectrometry was used to identify major antioxidant compounds and their content was determined by HPLC-DAD （detection wavelength of 320 nm）. Result： Oxyresveratrol was proved to be the major antioxidant compound in Mori Ramulus ethanolic extracts. Using the HPLC-DAD quantitative determination, Mori Ramulus oxyresveratrol had a good linear range of 14-1 260 mg . L-1 （r =0. 999 96）. The average recovery was 98.2% with RSD of 1.2%. This method is simple, rapid, accurate and sensitive. There was rich oxyrcsveratrol in Mori Ramulus and the content was significantly different according to the mulberry varieties. Conclusion ： The on-line HPLC-MS-DPPH method is applicable for the determination of antioxidant compounds in Mori Ramulns.