HPLC同时测定四逆汤中4种指标性成分的含量

Simultaneous determination of four components in Sini Tang by HPLC

目的:建立四逆汤中苯甲酰新乌头原碱、甘草苷、甘草酸和6-姜酚4种成分的高效液相色谱含量测定方法。方法:采用Thermo Hypersil BDS色谱柱(4.6 mm×250 mm,5μm),梯度洗脱,流速1.0 mL.min-1,检测波长235 nm。结果:苯甲酰新乌头原碱、甘草苷、甘草酸、6-姜酚均达到良好分离,线性范围分别为0.006～0.12(r=0.999 9),0.021～0.42(r=0.999 7),0.012～0.24(r=0.999 4),0.018～0.36 g.L-1(r=0.999 5);平均回收率分别为99.3%(RSD 1.5%),96.9%(RSD0.60%),100%(RSD 1.3%),100%(RSD 2.1%)。结论:该方法简便、准确、实用性强,可用于四逆汤中4种指标性成分的含量测定。

Objective： To develop an HPLC method for detelmining four components in Sini Tan, benzoylmesaconine, liquiritin, glycyrrhizie acid and 6-gingerol. Method： The Hypersil BDS column was adopted with gradient elntion program at a flow rate of 1.0 mL . min- 1 and the detection wavelength of 235 nm. Result： Benzoylmesaconine, liquiritin, glycyrrhizic acid and 6-gingerol showed good separation, with the linear range of 0. 006-0. 12, 0. 021-0. 42, 0. 012-0. 24 and 0. 018-0. 36 g . L-1 , respectively. Their average recoveries were 99. 3% , 96. 9% , 100% and 100% , respectively; and RSD of the above four components were 1.5% , 0. 6%, 1.3% and 2.1% , respectively. Conclusion： The method is proved to be so easy and accurate and practical that it can he used to determine the four components in Sini Tang.