摘要
目的:建立四逆汤中苯甲酰新乌头原碱、甘草苷、甘草酸和6-姜酚4种成分的高效液相色谱含量测定方法。方法:采用Thermo Hypersil BDS色谱柱(4.6 mm×250 mm,5μm),梯度洗脱,流速1.0 mL.min-1,检测波长235 nm。结果:苯甲酰新乌头原碱、甘草苷、甘草酸、6-姜酚均达到良好分离,线性范围分别为0.006~0.12(r=0.999 9),0.021~0.42(r=0.999 7),0.012~0.24(r=0.999 4),0.018~0.36 g.L-1(r=0.999 5);平均回收率分别为99.3%(RSD 1.5%),96.9%(RSD0.60%),100%(RSD 1.3%),100%(RSD 2.1%)。结论:该方法简便、准确、实用性强,可用于四逆汤中4种指标性成分的含量测定。
Objective: To develop an HPLC method for detelmining four components in Sini Tan, benzoylmesaconine, liquiritin, glycyrrhizie acid and 6-gingerol. Method: The Hypersil BDS column was adopted with gradient elntion program at a flow rate of 1.0 mL . min- 1 and the detection wavelength of 235 nm. Result: Benzoylmesaconine, liquiritin, glycyrrhizic acid and 6-gingerol showed good separation, with the linear range of 0. 006-0. 12, 0. 021-0. 42, 0. 012-0. 24 and 0. 018-0. 36 g . L-1 , respectively. Their average recoveries were 99. 3% , 96. 9% , 100% and 100% , respectively; and RSD of the above four components were 1.5% , 0. 6%, 1.3% and 2.1% , respectively. Conclusion: The method is proved to be so easy and accurate and practical that it can he used to determine the four components in Sini Tang.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2012年第6期803-805,共3页
China Journal of Chinese Materia Medica
基金
国家中医药管理局中医药行业科研专项(201007010)