原代SD大鼠海马神经元扫描电镜形态学特点

Characteristics of SD Rat Hippocampal Neuron by Scanning Electron Microscopy

目的:观察体外培养的原代海马神经元扫描电镜形态学特点。方法:体外培养新生SD乳鼠原代海马神经元,行免疫细胞化学鉴定,用扫描电镜观察其形态学特点。结果:培养的海马神经元形态多样,梭形神经元胞体较大,伸出两个突起;神经元细胞有的基底宽广,突起有粗有细,与周围的神经元细胞连结紧密;锥体细胞从胞体发出2～3个突起,从粗大的突起上又分出突起;培养的神经元以锥体形为主,其次为梭形、三角形。结论:培养的神经元为MAP-2染色阳性,扫描电镜可见神经元胞体及突起形成神经元的特有结构。

Objective： To observe morphological characteristics of primary SD rat hippocampal neuron with scanning electron microscopy.Methods： New born SD puppies was cultivated,with immunohistochemistry identification,and its morphological characteristics were observed with scanning electron microscopy.Results： Pimary cultivated neurons showed different shapes.Neuron body is larger,with 2 processes form it.Some cell bodies have broad bases,with p either thin processes or thick ones,and have a tight connection with around cell.Pyramid cells have 2-3 processes from cell bodies,with branches from those thicker ones.Cultivated cell mostly are pyramid cells,then spindle-shaped ones and triangle ones.Conclusion： Neurons are MAP-2 positive and scanning electron microscopy shows that cell body and process have special characteristics of neuron.