慢传输型便秘患者α-突触核蛋白硝基化的意义

Expression of nitrated α-synuclein in colon and its significance in patients with slow transit constipation

目的探讨α-突触核蛋白(α-Syn)在结肠慢传输型便秘(STC)中的变化及病理学意义。方法应用免疫荧光双染技术和病理显微分析图像系统,在中倍光镜下比较22例STC患者(STC组)和20例健康志愿者(对照组)结肠黏膜α-Syn及硝基化α-Syn。结果两组结肠黏膜神经元及胶质细胞均有α-Syn及硝基化α-Syn表达。STC组结肠黏膜α-Syn及硝基化α-Syn较对照组明显增加,α-Syn黏膜层1101.1±26.34 vs.1431.3±30.36;黏膜下1023.1±31.42vs.1332.7±26.3;硝基化α-Syn(黏膜层396±14.16 vs.688.5±28.34;黏膜下129.7±3.78 vs.220.9±8.55),差异有显著性(P<0.05)。结论结肠黏膜神经α-Syn的硝基化参与STC的发病。

Objective To study the expression of nitrated alpha-synuclein（α-Syn） in patients with slow transit constipation（STC）,and to explore its role in pathogenesis of slow transit constipation（STC）.Methods By using double-labeled immunofluorescent staining,the expression and distribution of α-Syn and nitrated α-Syn in colon of 20 patients with STC and 20 healthy controls were studied.Results α-Syn and nitrated α-Syn were positively scattered in neurons and fibers of submucosa and mucosa layers.The positive scores in STC group were significantly higher than those of controls [（α-Syn in mucosal layer 1101.1±26.34 vs.1431.3±30.36;and in submucosa 1023.1±31.42 vs.1332.7±26.3）;（nitrated α-Syn in mucosal layer 396±14.16 vs.688.5±28.34;and in submucosa 129.7±3.78 vs.220.9±8.55）,（P0.05）].Conclusion The expression of nitrated α-Syn is increased in colon of STC patients,thus it may possibly be contributed to the pathogenesis of STC.