摘要
目的:选用荷瘤小鼠作为研究对象,观察大黄虫丸对肿瘤细胞的抑瘤和诱导凋亡作用,旨在探讨大黄虫丸抗肿瘤效应机制,为其临床应用和创制新药提供理论依据。方法:建立小鼠克洛氏(S180)肉瘤动物模型,以大黄虫丸方82g/kg剂量连续灌胃10天,同时设模型对照组、正常组和西药(环磷酰胺)对照组,取治疗组、对照组及模型组实体瘤分别涂片观察肿瘤细胞中Survinvin的表达。结果:与模型对照组及西药(环磷酰胺)对照组比较,大黄虫丸治疗组肿瘤组织中Survinvin的表达明显降低。结论:大黄虫丸能够调低肿瘤细胞中Survinvin的表达,诱导肿瘤的凋亡。
Obejective ： Using tumor - bearing mice as targets, we investigate the inhibitory and apoptosis - inducing effects on tumor cell of Dahuang Zhechong Pill to study its anti - tumor effect mechanism, and to provide theoretical basis for its clinical and pharmaceutical applications. Methods：After building a mice bearing S180 sarcoma model, we carry out oral tests in mice in an 82g/kg dose for 10 days. A model control group and a cyclophosphamide control group have also been built. The survinvin expressions in cancer cells for all groups have been observed by smear tests of tumors. Results ： Dahuang Zhechong Pill group has much more significant inhibitory effect on survinvin expression compared with the model control group as well as cyclophosphamide control group. Conclusion：Dahuang Zhechong Pill can inhibit the survinvin expression in cancer cell and induce tumor apoptosis.
出处
《中华中医药学刊》
CAS
2012年第3期462-463,I0001,共3页
Chinese Archives of Traditional Chinese Medicine
基金
辽宁省教育厅资助项目(200908)
